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Literature summary for 1.4.3.21 extracted from

  • Atkin, K.E.; Reiss, R.; Turner, N.J.; Brzozowski, A.M.; Grogan, G.
    Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of variants of monoamine oxidase from Aspergillus niger (2008), Acta Crystallogr. Sect. F, 64, 182-185.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.3.21

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of His-tagged wild-type and mutants enzymes in Escherichia coli strain Rosetta2(DE3) Aspergillus niger

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant genetic variants MAO-N-3 and MAO-N-5, from 10% w/v PEG 3350, 0.2 M proline, 0.1 M HEPES, pH 7.5, or 10% w/v PEG 5000 MME, 5% v/v tacsimate, 0.1 M HEPES, pH 7.0, with no difference in diffraction quality between the crystals from the two conditions, X-ray diffraction structure determination and analysis at 2.45 A and 1.85 A resolution, respectively Aspergillus niger

Protein Variants

Protein Variants Comment Organism
additional information genetic variants of MAO-N produced by directed evolution possess altered substrate specificity, e.g. MAO-N-3 and MAO-N-5 Aspergillus niger
N336S/M348K/I246M genetic variant MOA-N-3 exhibits improved activity towards a range of amine substrates compared to the wild-type enzyme, including chiral secondary amines Aspergillus niger
N336S/M348K/I246M/T384N/D385S genetic variant MOA-N-5 shows improved activity and enantioselectivity towards a broad range of tertiary amines compared to the wild-type enzyme Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
rasagiline ethanedisulfonate inhibits MAO-B Aspergillus niger
tacsimate
-
 Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutants enzymes from Escherichia coli strain Rosetta2(DE3), removal of the His tag Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information MAO-N is an FAD-dependent enzyme that catalyses the conversion of terminal amines to their corresponding aldehyde Aspergillus niger ?
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Aspergillus niger

Synonyms

Synonyms Comment Organism
MAO-N
-
 Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
FAD dependent on Aspergillus niger