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Literature summary for 1.4.3.21 extracted from

  • Mills, S.A.; Klinman, J.P.
    Evidence against reduction of Cu2+ to Cu+ during dioxygen activation in a copper amine oxidase from Yeast (2000), J. Am. Chem. Soc., 122, 9897-9904.
No PubMed abstract available
Search for more literature for 1.4.3.21

Cloned(Commentary)

Cloned (Comment) Organism
-
 Ogataea angusta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 methylamine native enzyme Ogataea angusta
0.68
-
 methylamine Co2+ reconstituted enzyme Ogataea angusta

Metals/Ions

Metals/Ions Comment Organism Structure
copper copper depleted enzyme can be reconstituted with either Cu2+, Zn2+, Co2+, or Ni2+, 79% of activity is restored with Cu2+, 19% is restored with Co2+, 1.7% with Zn2+ or Ni2+ Ogataea angusta

Organism

Organism UniProt Comment Textmining
Ogataea angusta
-
 1A2V
-
Ogataea angusta 1A2V
-
 1A2V
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ogataea angusta

Reaction

Reaction Comment Organism Reaction ID
RCH2NH2 + H2O + O2 = RCHO + NH3 + H2O2 role of copper in enzyme activity Ogataea angusta
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0193
-
 copper depleted, reconstituted with Zn2+ Ogataea angusta
0.0195
-
 copper depleted, reconstituted with Ni2+ Ogataea angusta
0.0571
-
 copper depleted enzyme Ogataea angusta
0.213
-
 copper depleted, reconstituted with Co2+ Ogataea angusta
0.894
-
 copper depleted, reconstituted with Cu2+ Ogataea angusta
1.13
-
 native enzyme Ogataea angusta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methylamine + H2O + O2
-
 Ogataea angusta methanal + NH3 + H2O2
-
 ?
methylamine + H2O + O2
-
 Ogataea angusta 1A2V methanal + NH3 + H2O2
-
 ?
RCH2NH2 + H2O + O2
-
 Ogataea angusta RCHO + NH3 + H2O2
-
 ?
RCH2NH2 + H2O + O2
-
 Ogataea angusta 1A2V RCHO + NH3 + H2O2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.08
-
 methylamine Co2+ reconstituted enzyme Ogataea angusta
2.12
-
 methylamine native enzyme Ogataea angusta