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Literature summary for 1.4.3.20 extracted from
- Purification and partial characterization of marinocine, a new broad-spectrum antibacterial protein produced by Marinomonas mediterranea (2005), Biochim. Biophys. Acta, 1721, 193-203.
General Stability
| General Stability | Organism |
|---|---|
| quite resistant to hydrolytic enzymes | Marinomonas mediterranea |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 160000 | - |
gel filtration, non-denaturing | Marinomonas mediterranea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Marinomonas mediterranea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Marinomonas mediterranea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | at death phase of growth | Marinomonas mediterranea | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme shows antibacterial activity | Marinomonas mediterranea | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | two major bands of 97000 Da and 185000 Da appear in denaturing SDS-PAGE | Marinomonas mediterranea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| marinocine | - |
Marinomonas mediterranea |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
at pH 7, the incubation of the samples at temperatures below inactivation produces a characteristic and reliable increase in activity. This activation is already noticeable at 50°C for treatments longer than 1 h | Marinomonas mediterranea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
pH 5 and 7, 1 h, antibacterial activity is stable up to 70 | Marinomonas mediterranea |
| 75 | - |
heat exposure at temperatures up to 75°C and pH 7 cause a conformational change in the marinocine structure leading to this rare strong activation, but temperatures above 75°C cause the denaturation in marinocine clearly observed in the electrophoresis experiments, with appearance of the two bands of 97000 Da and 185000Da | Marinomonas mediterranea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
at pH 7, the incubation of the samples at temperatures below inactivation produces a characteristic and reliable increase in activity. This activation is already noticeable at 50°C for treatments longer than 1 h | Marinomonas mediterranea |
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