Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha,alpha'-dipyridyl | 119% activity at 1 mM (pH 7.0) | Pseudomonas sp. | |
KCN | enhances the lysine oxidase activity (177% activity at 1 mM (pH 5.5)) | Pseudomonas sp. | |
N-ethylmaleimide | remarkably enhances the lysine oxidase activity (500% activity at 1 mM (pH 7.0)) | Pseudomonas sp. | |
p-chloromercuribenzoic acid | remarkably enhances the lysine oxidase activity | Pseudomonas sp. | |
Sodium azide | 116% activity at 1 mM (pH 7.0) | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxyquinoline | 76% residual activity at 1 mM (pH 5.5) | Pseudomonas sp. | |
Co2+ | 67% residual activity at 1 mM (pH 5.5) | Pseudomonas sp. | |
EDTA | 80% residual activity at 1 mM (pH 5.5) | Pseudomonas sp. | |
Fe2+ | 62% residual activity at 1 mM (pH 5.5) | Pseudomonas sp. | |
Hg2+ | complete inhibition at 1 mM (pH 7.0) | Pseudomonas sp. | |
hydrazine | 46% residual activity at 1 mM (pH 5.5) | Pseudomonas sp. | |
hydroxylamine | 63% residual activity at 1 mM (pH 5.5) | Pseudomonas sp. | |
iodoacetic acid | 0.5% residual activity at 1 mM (pH 7.0) | Pseudomonas sp. | |
KCN | 70% residual activity at 1 mM (pH 7.0) | Pseudomonas sp. | |
Mg2+ | 78% residual activity at 1 mM (pH 5.5) | Pseudomonas sp. | |
Mn2+ | 63% residual activity at 1 mM (pH 5.5) | Pseudomonas sp. | |
Ni2+ | 55% residual activity at 1 mM (pH 5.5) | Pseudomonas sp. | |
o-phenanthroline | 15% residual activity at 1 mM (pH 5.5) | Pseudomonas sp. | |
phenylhydrazine | complete inhibition at 1 mM (pH 5.5) | Pseudomonas sp. | |
Semicarbazide | 32% residual activity at 1 mM (pH 5.5) | Pseudomonas sp. | |
Zn2+ | 24% residual activity at 1 mM (pH 5.5) | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54500 | - |
2 * 54500, SDS-PAGE | Pseudomonas sp. |
110000 | - |
gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-amino acid + H2O + O2 | Pseudomonas sp. | - |
2-oxocarboxylate + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Toyopearl column chromatography, phenyl Toyopearl column chromatography, and L-lysine-Toyopearl resin column chromatography | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.003 | - |
cell-free extract, using L-lysine as substrate, at pH 7.0 and 65°C | Pseudomonas sp. |
1.16 | - |
after 389fold purification, using L-lysine as substrate, at pH 7.0 and 65°C | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-amino acid + H2O + O2 | - |
Pseudomonas sp. | 2-oxocarboxylate + NH3 + H2O2 | - |
? | |
L-arginine + H2O + O2 | 6% activity compared to L-lysine | Pseudomonas sp. | 5-carbamidamido-2-oxopentanoate + NH3 + H2O2 | - |
? | |
L-lysine + H2O + O2 | 100% activity | Pseudomonas sp. | 6-amino-2-oxohexanoate + NH3 + H2O2 | - |
? | |
L-ornithine + H2O + O2 | 31% activity compared to L-lysine | Pseudomonas sp. | ? | - |
? | |
additional information | the enzyme does not oxidize other L-amino acids and D-amino acids besides L-lysine, L-ornithine and L-arginine | Pseudomonas sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 54500, SDS-PAGE | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
L-amino acid oxidase | - |
Pseudomonas sp. |
LAAO | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
temperature optimum for for oxidation of L-ornithine | Pseudomonas sp. |
50 | 60 | temperature optimum for for oxidation of L-arginine | Pseudomonas sp. |
65 | - |
temperature optimum for oxidation of L-lysine | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
pH optimum for oxidation of L-lysine | Pseudomonas sp. |
9 | - |
pH optimum for oxidation of L-ornithine and L-arginine | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas sp. | isoelectric focusing | - |
4.6 |
Organism | Comment | Expression |
---|---|---|
Pseudomonas sp. | the enzyme is remarkably induced by incubation with L-lysine as a nitrogen source | up |