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Literature summary for 1.4.3.2 extracted from

  • Stasyk, T.; Lutsik-Kordovsky, M.; Wernstedt, C.; Antonyuk, V.; Klyuchivska, O.; Souchelnytskyi, S.; Hellman, U.; Stoika, R.
    A new highly toxic protein isolated from the death cap Amanita phalloides is an L-amino acid oxidase (2010), FEBS J., 277, 1260-1269.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by caspase III inhibitor benzyloxycarbonyl-Asp(OMe)-fluoromethylketone Amanita phalloides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DL-asparagine + H2O + O2 Amanita phalloides low activity 2-oxosuccinamic acid + NH3 + H2O2
-
?
DL-leucine + H2O + O2 Amanita phalloides
-
2-oxo-4-methylpentanoate + NH3 + H2O2
-
?
DL-methionine + H2O + O2 Amanita phalloides
-
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
?
DL-norleucine + H2O + O2 Amanita phalloides
-
2-oxocaproate + NH3 + H2O2
-
?
DL-threonine + H2O + O2 Amanita phalloides low activity 2-oxo-3-hydroxybutanoic acid + NH3 + H2O2
-
?
DL-tryptophan + H2O + O2 Amanita phalloides low activity 3-(1H-indol-3-yl)-2-oxopropanoic acid + NH3 + H2O2
-
?
DL-valine + H2O + O2 Amanita phalloides low activity 2-oxoisovalerate + NH3 + H2O2
-
?
L-arginine + H2O + O2 Amanita phalloides
-
2-oxo-5-guanidinopentanoic acid + NH3 + H2O2
-
?
L-glutamic acid + H2O + O2 Amanita phalloides low activity 2-oxoglutarate + NH3 + H2O2
-
?
L-histidine + H2O + O2 Amanita phalloides low activity 2-oxo-4-imidazolepropionic acid + NH3 + H2O2
-
?
L-isoleucine + H2O + O2 Amanita phalloides
-
3-methyl-2-oxopentanoate + NH3 + H2O2
-
?
L-lysine + H2O + O2 Amanita phalloides low activity 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
?
L-methionine + H2O + O2 Amanita phalloides preferred substrate 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
?
L-phenylalanine + H2O + O2 Amanita phalloides
-
phenylpyruvate + NH3 + H2O2
-
?
L-tyrosine + H2O + O2 Amanita phalloides
-
4-hydroxyphenylpyruvate + NH3 + H2O2
-
?
lysine + H2O + O2 Amanita phalloides low activity 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
?
additional information Amanita phalloides toxophallin induces chromatin condensation, as well as DNA and nucleus fragmentation, which are typical for apoptosis, effect towards human leukemia CEM-T4 and murine leukemia L1210 cells, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Amanita phalloides
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit body
-
Amanita phalloides
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-asparagine + H2O + O2 low activity Amanita phalloides 2-oxosuccinamic acid + NH3 + H2O2
-
?
DL-leucine + H2O + O2
-
Amanita phalloides 2-oxo-4-methylpentanoate + NH3 + H2O2
-
?
DL-methionine + H2O + O2
-
Amanita phalloides 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
?
DL-norleucine + H2O + O2
-
Amanita phalloides 2-oxocaproate + NH3 + H2O2
-
?
DL-threonine + H2O + O2 low activity Amanita phalloides 2-oxo-3-hydroxybutanoic acid + NH3 + H2O2
-
?
DL-tryptophan + H2O + O2 low activity Amanita phalloides 3-(1H-indol-3-yl)-2-oxopropanoic acid + NH3 + H2O2
-
?
DL-valine + H2O + O2 low activity Amanita phalloides 2-oxoisovalerate + NH3 + H2O2
-
?
L-arginine + H2O + O2
-
Amanita phalloides 2-oxo-5-guanidinopentanoic acid + NH3 + H2O2
-
?
L-glutamic acid + H2O + O2 low activity Amanita phalloides 2-oxoglutarate + NH3 + H2O2
-
?
L-histidine + H2O + O2 low activity Amanita phalloides 2-oxo-4-imidazolepropionic acid + NH3 + H2O2
-
?
L-isoleucine + H2O + O2
-
Amanita phalloides 3-methyl-2-oxopentanoate + NH3 + H2O2
-
?
L-lysine + H2O + O2 low activity Amanita phalloides 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
?
L-methionine + H2O + O2 preferred substrate Amanita phalloides 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
?
L-phenylalanine + H2O + O2
-
Amanita phalloides phenylpyruvate + NH3 + H2O2
-
?
L-tyrosine + H2O + O2
-
Amanita phalloides 4-hydroxyphenylpyruvate + NH3 + H2O2
-
?
lysine + H2O + O2 low activity Amanita phalloides 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
?
additional information toxophallin induces chromatin condensation, as well as DNA and nucleus fragmentation, which are typical for apoptosis, effect towards human leukemia CEM-T4 and murine leukemia L1210 cells, overview Amanita phalloides ?
-
?
additional information L-cysteine, L-glycine, L-proline, L-oxyproline, DL-serine, and DL-aspartic acid are no substrates. Toxophallin is also not active against benzylamine, ethanolamine, diethylamine, meta- and para-phenylendiamine, ortho-, meta- and paraaminophenols, or putrescin Amanita phalloides ?
-
?

Subunits

Subunits Comment Organism
More toxophallin peptide mapping and sequence analysis by MALDI-TOF mass spectrometry, overview Amanita phalloides

Synonyms

Synonyms Comment Organism
toxophallin
-
Amanita phalloides

General Information

General Information Comment Organism
additional information toxophallin is a highly cytotoxic protein, but differs distinctly from the other death cap toxic protein phallolysin. The interaction of toxophallin with target cells is not mediated by a specific cell surface receptor Amanita phalloides
physiological function toxophallin induces chromatin condensation, as well as DNA and nucleus fragmentation, which are typical for apoptosis. Toxophallin uses a caspase-independent pathway of apoptosis induction Amanita phalloides