Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.3.2 extracted from

  • Rodrigues, R.S.; da Silva, J.F.; Boldrini Franca, J.; Fonseca, F.P.; Otaviano, A.R.; Henrique Silva, F.; Hamaguchi, A.; Magro, A.J.; Braz, A.S.; dos Santos, J.I.; Homsi-Brandeburgo, M.I.; Fontes, M.R.; Fuly, A.L.; Soares, A.M.; Rodrigues, V.M.
    Structural and functional properties of Bp-LAAO, a new L-amino acid oxidase isolated from Bothrops pauloensis snake venom (2009), Biochimie, 91, 490-501.
    View publication on PubMed
Search for more literature for 1.4.3.2

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis Bothrops pauloensis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular venom Bothrops pauloensis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 2 * 65000, SDS-PAGE, 2 * 58000, about, sequence calculation Bothrops pauloensis
65000
-
 2 * 65000, SDS-PAGE, 2 * 58000, about, sequence calculation Bothrops pauloensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bothrops pauloensis LAAO shows leishmanicidal, antitumoral and bactericidal activities dose dependently, overview. LAAO induces platelet aggregation in platelet-rich plasma which is inhibited by catalase ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bothrops pauloensis B5AR80
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
flavoprotein
-
 Bothrops pauloensis
glycoprotein
-
 Bothrops pauloensis

Purification (Commentary)

Purification (Comment) Organism
native enzyme from venom by cation exchange and hydrophobic interaction chromatography, followed by adosrption chromatography and HPLC Bothrops pauloensis

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Bothrops pauloensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate specificity, overview Bothrops pauloensis
60
-
 purified enzyme, substrate L-Ala Bothrops pauloensis
110
-
 purified enzyme, substrate L-Val Bothrops pauloensis
140
-
 purified enzyme, substrates L-Tyr or L-Glu Bothrops pauloensis
310
-
 purified enzyme, substrate L-Trp Bothrops pauloensis
335
-
 purified enzyme, substrate L-His Bothrops pauloensis
340
-
 purified enzyme, substrate L-Arg Bothrops pauloensis
480
-
 purified enzyme, substrate L-Ile Bothrops pauloensis
545
-
 purified enzyme, substrate L-Phe Bothrops pauloensis
575
-
 purified enzyme, substrate L-Leu Bothrops pauloensis
580
-
 purified enzyme, substrate L-Met Bothrops pauloensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala + H2O + O2
-
 Bothrops pauloensis 2-oxopropanoic acid + NH3 + H2O2
-
 ?
L-Arg + H2O + O2
-
 Bothrops pauloensis 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
 ?
L-Glu + H2O + O2
-
 Bothrops pauloensis 2-oxoglutarate + NH3 + H2O2
-
 ?
L-His + H2O + O2
-
 Bothrops pauloensis 3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
-
 ?
L-Ile + H2O + O2
-
 Bothrops pauloensis 3-methyl-2-oxopentanoate + NH3 + H2O2
-
 ?
L-Leu + H2O + O2
-
 Bothrops pauloensis 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
L-Met + H2O + O2 best substrate Bothrops pauloensis 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
L-Phe + H2O + O2
-
 Bothrops pauloensis phenylpyruvate + NH3 + H2O2
-
 ?
L-Trp + H2O + O2
-
 Bothrops pauloensis indol-3-pyruvate + NH3 + H2O2
-
 ?
L-Tyr + H2O + O2
-
 Bothrops pauloensis 4-hydroxyphenylpyruvate + NH3 + H2O2
-
 ?
L-Val + H2O + O2
-
 Bothrops pauloensis 2-oxoisovalerate + NH3 + H2O2
-
 ?
additional information LAAO shows leishmanicidal, antitumoral and bactericidal activities dose dependently, overview. LAAO induces platelet aggregation in platelet-rich plasma which is inhibited by catalase Bothrops pauloensis ?
-
 ?
additional information substrate specificity, no activity with L-Pro, L-Thr, L-Ser, and L-Cys, overview. Construction of a LAAO structural model of the stereochemistry of substrate binding by the enzyme, overview Bothrops pauloensis ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 65000, SDS-PAGE, 2 * 58000, about, sequence calculation Bothrops pauloensis
More structure analysis and molecular dynamics simulation, overview Bothrops pauloensis

Synonyms

Synonyms Comment Organism
LAAO
-
 Bothrops pauloensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Bothrops pauloensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Bothrops pauloensis

Cofactor

Cofactor Comment Organism Structure
FAD a flavoenzyme Bothrops pauloensis

pI Value

Organism Comment pI Value Maximum pI Value
Bothrops pauloensis sequence calculation
-
 6.3