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Literature summary for 1.4.3.2 extracted from

  • Tonismaegi, K.; Samel, M.; Trummal, K.; Roennholm, G.; Siigur, J.; Kalkkinen, N.; Siigur, E.
    L-amino acid oxidase from Vipera lebetina venom: isolation, characterization, effects on platelets and bacteria (2006), Toxicon, 48, 227-237.
    View publication on PubMed
Search for more literature for 1.4.3.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 L-tryptophan pH 8.5, 25°C Macrovipera lebetina
0.4
-
 L-leucine pH 8.5, 25°C Macrovipera lebetina
0.65
-
 L-methionine pH 8.5, 25°C Macrovipera lebetina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60900
-
 2 * 60900, MALDI-TOF, 2 * 66000, SDS-PAGE Macrovipera lebetina
66000
-
 2 * 60900, MALDI-TOF, 2 * 66000, SDS-PAGE Macrovipera lebetina
140000
-
 or above, gel filtration Macrovipera lebetina

Organism

Organism UniProt Comment Textmining
Macrovipera lebetina
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Macrovipera lebetina

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Macrovipera lebetina
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8
-
 substrate L-isoleucine, pH 8.5, 25°C Macrovipera lebetina
4.5
-
 substrate L-arginine, pH 8.5, 25°C Macrovipera lebetina
4.5
-
 substrate L-phenylalanine, pH 8.5, 25°C Macrovipera lebetina
5.5
-
 substrate L-histidine, pH 8.5, 25°C Macrovipera lebetina
6.5
-
 substrate L-leucine, pH 8.5, 25°C Macrovipera lebetina
7.2
-
 substrate L-tryptophan, pH 8.5, 25°C Macrovipera lebetina
14.4
-
 substrate L-methionine, pH 8.5, 25°C Macrovipera lebetina

Storage Stability

Storage Stability Organism
-20°C, loss of 80% of activity Macrovipera lebetina
25°C, stable for at least 24h Macrovipera lebetina
4°C, stable for at least 24h Macrovipera lebetina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O + O2 substrate affinity in decreasing order: L-methionine, L-tryptophan, L-leucine, L-histidine, L-phenylalanine, L-arginine, L-isoleucine Macrovipera lebetina 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
 ?
L-histidine + H2O + O2 substrate affinity in decreasing order: L-methionine, L-tryptophan, L-leucine, L-histidine, L-phenylalanine, L-arginine, L-isoleucine Macrovipera lebetina 3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
-
 ?
L-isoleucine + H2O + O2 substrate affinity in decreasing order: L-methionine, L-tryptophan, L-leucine, L-histidine, L-phenylalanine, L-arginine, L-isoleucine Macrovipera lebetina 3-methyl-2-oxo-pentanoate + NH3 + H2O2
-
 ?
L-leucine + H2O + O2 substrate affinity in decreasing order: L-methionine, L-tryptophan, L-leucine, L-histidine, L-phenylalanine, L-arginine, L-isoleucine Macrovipera lebetina 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
L-methionine + H2O + O2 substrate affinity in decreasing order: L-methionine, L-tryptophan, L-leucine, L-histidine, L-phenylalanine, L-arginine, L-isoleucine Macrovipera lebetina 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
L-phenylalanine + H2O + O2 substrate affinity in decreasing order: L-methionine, L-tryptophan, L-leucine, L-histidine, L-phenylalanine, L-arginine, L-isoleucine Macrovipera lebetina phenylpyruvate + NH3 + H2O2
-
 ?
L-tryptophan + H2O + O2 substrate affinity in decreasing order: L-methionine, L-tryptophan, L-leucine, L-histidine, L-phenylalanine, L-arginine, L-isoleucine Macrovipera lebetina 3-indole-2-oxopropanoate + NH3 + H2O2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 60900, MALDI-TOF, 2 * 66000, SDS-PAGE Macrovipera lebetina

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 15 min, complete inactivation Macrovipera lebetina

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.21
-
 L-methionine pH 8.5, 25°C Macrovipera lebetina
42.6
-
 L-tryptophan pH 8.5, 25°C Macrovipera lebetina
52
-
 L-leucine pH 8.5, 25°C Macrovipera lebetina
80.3
-
 L-methionine pH 8.5, 25°C Macrovipera lebetina