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Literature summary for 1.4.3.19 extracted from
- Asn336 is involved in the substrate affinity of glycine oxidase from Bacillus cereus (2016), Electron. J. Biotechnol., 22, 26-30 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Bacillus cereus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N336A | the mutant shows increased substrate affinity compared to the wild type enzyme | Bacillus cereus |
| N336G | the mutant shows increased substrate affinity compared to the wild type enzyme | Bacillus cereus |
| N336H | the mutant shows increased substrate affinity compared to the wild type enzyme | Bacillus cereus |
| N336K | the mutant shows increased substrate affinity compared to the wild type enzyme | Bacillus cereus |
| N336R | the mutant shows increased substrate affinity compared to the wild type enzyme | Bacillus cereus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.53 | - |
glycine | mutant enzyme N336R, at pH 7.5, temperature not specified in the publication | Bacillus cereus |  |
| 0.79 | - |
glycine | mutant enzyme N336K, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.95 | - |
glycine | mutant enzyme N336H, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 1.04 | - |
glycine | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 1.41 | - |
glycine | mutant enzyme N336A, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 1.51 | - |
sarcosine | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 2.25 | - |
glycine | mutant enzyme N336G, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 10.44 | - |
glyphosate | mutant enzyme N336R, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 22.45 | - |
glyphosate | mutant enzyme N336K, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 34.65 | - |
D-alanine | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 42.31 | - |
glyphosate | mutant enzyme N336H, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 68.36 | - |
glyphosate | mutant enzyme N336A, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 84.79 | - |
glyphosate | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 96.73 | - |
glyphosate | mutant enzyme N336G, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycine + H2O + O2 | Bacillus cereus | - |
glyoxylate + NH3 + H2O2 | - |
? | |
| glycine + H2O + O2 | Bacillus cereus HYC-7 | - |
glyoxylate + NH3 + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | L7T8Y9 | - |
- |
| Bacillus cereus HYC-7 | L7T8Y9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| GST-Bind resin column chromatography and Superdex 200 gel filtration | Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-alanine + H2O + O2 | - |
Bacillus cereus | ? | - |
? | |
| D-alanine + H2O + O2 | - |
Bacillus cereus HYC-7 | ? | - |
? | |
| glycine + H2O + O2 | - |
Bacillus cereus | glyoxylate + NH3 + H2O2 | - |
? | |
| glycine + H2O + O2 | - |
Bacillus cereus HYC-7 | glyoxylate + NH3 + H2O2 | - |
? | |
| glyphosate + H2O + O2 | - |
Bacillus cereus | ? | - |
? | |
| glyphosate + H2O + O2 | - |
Bacillus cereus HYC-7 | ? | - |
? | |
| sarcosine + H2O + O2 | - |
Bacillus cereus | ? | - |
? | |
| sarcosine + H2O + O2 | - |
Bacillus cereus HYC-7 | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.021 | - |
glyphosate | mutant enzyme N336R, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.024 | - |
glycine | mutant enzyme N336R, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.19 | - |
glyphosate | mutant enzyme N336K, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.23 | - |
glyphosate | mutant enzyme N336H, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.25 | - |
glycine | mutant enzyme N336K, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.37 | - |
glycine | mutant enzyme N336H, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.52 | - |
glycine | mutant enzyme N336A, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.62 | - |
glyphosate | mutant enzyme N336G, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.67 | - |
glyphosate | mutant enzyme N336A, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.67 | - |
glycine | mutant enzyme N336G, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.71 | - |
glycine | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.81 | - |
D-alanine | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.87 | - |
glyphosate | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.98 | - |
sarcosine | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
glyphosate | mutant enzyme N336R, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.0054 | - |
glyphosate | mutant enzyme N336H, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.0064 | - |
glyphosate | mutant enzyme N336G, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.0084 | - |
glyphosate | mutant enzyme N336K, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.0098 | - |
glyphosate | mutant enzyme N336A, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.01 | - |
glyphosate | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.023 | - |
D-alanine | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.045 | - |
glycine | mutant enzyme N336R, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.3 | - |
glycine | mutant enzyme N336G, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.32 | - |
glycine | mutant enzyme N336K, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.37 | - |
glycine | mutant enzyme N336A, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.39 | - |
glycine | mutant enzyme N336H, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.65 | - |
sarcosine | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
| 0.68 | - |
glycine | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
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