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Literature summary for 1.4.3.14 extracted from

  • Arinbasarova, A.; Ashin, V.; Makrushin, K.; Medentsev, A.; Lukasheva, E.; Berezov, T.
    Isolation and properties of L-lysine-alpha-oxidase from the fungus Trichoderma cf. aureoviride RIFAI VKM F-4268D (2012), Microbiology, 81, 549-554.
    View publication on PubMed
Search for more literature for 1.4.3.14

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000 116000 gel filtration Trichoderma cf. aureoviride

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arginine + O2 + H2O Trichoderma cf. aureoviride 8.3% activity compared to L-lysine 5-carbamidamido-2-oxopentanoate + NH3 + H2O2
-
 ?
L-arginine + O2 + H2O Trichoderma cf. aureoviride Rifai VKM F-4268D 8.3% activity compared to L-lysine 5-carbamidamido-2-oxopentanoate + NH3 + H2O2
-
 ?
L-lysine + O2 + H2O Trichoderma cf. aureoviride the stereospecific enzyme oxidizes almost exclusively L-lysine 6-amino-2-oxohexanoate + NH3 + H2O2
-
 ?
L-lysine + O2 + H2O Trichoderma cf. aureoviride Rifai VKM F-4268D the stereospecific enzyme oxidizes almost exclusively L-lysine 6-amino-2-oxohexanoate + NH3 + H2O2
-
 ?
L-ornithine + O2 + H2O Trichoderma cf. aureoviride 5.8% activity compared to L-lysine 5-amino-2-oxopentanoate + NH3 + H2O2
-
 ?
L-ornithine + O2 + H2O Trichoderma cf. aureoviride Rifai VKM F-4268D 5.8% activity compared to L-lysine 5-amino-2-oxopentanoate + NH3 + H2O2
-
 ?
additional information Trichoderma cf. aureoviride no activity with The L-isomers of leucine, phenylalanine, citrulline, alanine, asparagine, histidine, ornithine, glutamine, threonine, tyrosine, isoleucine, valine, as well as glycine and D-lysine ?
-
 ?
additional information Trichoderma cf. aureoviride Rifai VKM F-4268D no activity with The L-isomers of leucine, phenylalanine, citrulline, alanine, asparagine, histidine, ornithine, glutamine, threonine, tyrosine, isoleucine, valine, as well as glycine and D-lysine ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trichoderma cf. aureoviride
-
-
-
Trichoderma cf. aureoviride Rifai VKM F-4268D
-
-
-

Purification (Commentary)

Purification (Comment) Organism
octyl Sepharose column chromatography, DEAE ToyoPearl column chromatography, and gel filtration Trichoderma cf. aureoviride

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Trichoderma cf. aureoviride
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.29
-
 culture liquid, at pH 8.0 and 25°C Trichoderma cf. aureoviride
90
-
 after 310fold purification, at pH 8.0 and 25°C Trichoderma cf. aureoviride

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + O2 + H2O 8.3% activity compared to L-lysine Trichoderma cf. aureoviride 5-carbamidamido-2-oxopentanoate + NH3 + H2O2
-
 ?
L-arginine + O2 + H2O 8.3% activity compared to L-lysine Trichoderma cf. aureoviride Rifai VKM F-4268D 5-carbamidamido-2-oxopentanoate + NH3 + H2O2
-
 ?
L-lysine + O2 + H2O the stereospecific enzyme oxidizes almost exclusively L-lysine Trichoderma cf. aureoviride 6-amino-2-oxohexanoate + NH3 + H2O2
-
 ?
L-lysine + O2 + H2O the stereospecific enzyme oxidizes almost exclusively L-lysine Trichoderma cf. aureoviride Rifai VKM F-4268D 6-amino-2-oxohexanoate + NH3 + H2O2
-
 ?
L-ornithine + O2 + H2O 5.8% activity compared to L-lysine Trichoderma cf. aureoviride 5-amino-2-oxopentanoate + NH3 + H2O2
-
 ?
L-ornithine + O2 + H2O 5.8% activity compared to L-lysine Trichoderma cf. aureoviride Rifai VKM F-4268D 5-amino-2-oxopentanoate + NH3 + H2O2
-
 ?
additional information no activity with The L-isomers of leucine, phenylalanine, citrulline, alanine, asparagine, histidine, ornithine, glutamine, threonine, tyrosine, isoleucine, valine, as well as glycine and D-lysine Trichoderma cf. aureoviride ?
-
 ?
additional information no activity with The L-isomers of leucine, phenylalanine, citrulline, alanine, asparagine, histidine, ornithine, glutamine, threonine, tyrosine, isoleucine, valine, as well as glycine and D-lysine Trichoderma cf. aureoviride Rifai VKM F-4268D ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 57000-58000, SDS-PAGE Trichoderma cf. aureoviride

Synonyms

Synonyms Comment Organism
L-lysine-alpha-oxidase
-
 Trichoderma cf. aureoviride

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 the enzyme is stable at temperatures up to 50°C Trichoderma cf. aureoviride

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.2
-
 Trichoderma cf. aureoviride

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 about 50% activity at pH 6.0, about 80% activity at pH 7.0, 100% activity at pH 8.0, about 85% activity at pH 9.0, about 78% activity at pH 9.5 Trichoderma cf. aureoviride

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Trichoderma cf. aureoviride

Expression

Organism Comment Expression
Trichoderma cf. aureoviride enzyme expression is induced in submerged culture on wheat bran under salt stress conditions (6% (w/v) NaCl) up