Cloned (Comment) | Organism |
---|---|
expression of proteolytically processed forms of LOX, LOXL1, and LOXL2 in Escherichia coli as N-terminally His6-tagged proteins | Homo sapiens |
General Stability | Organism |
---|---|
sucrose, sorbitol, and mannitol are less effective in stabilization and preservation of the enzyme compared to trehalose | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-aminopropionitrile | inhibition of active recombinant LOX, LOXL1, and LOXL2, specific | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | dependent on, copper-binding domain in LOX, LOXL1, LOXL2, LOXL3, and LOXL4 | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 36000, recombinant processed LOX, SDS-PAGE, x * 51000, recombinant processed LOXL1, SDS-PAGE, x * 29000, recombinant processed LOXL2, SDS-PAGE | Homo sapiens |
36000 | - |
x * 36000, recombinant processed LOX, SDS-PAGE, x * 51000, recombinant processed LOXL1, SDS-PAGE, x * 29000, recombinant processed LOXL2, SDS-PAGE | Homo sapiens |
51000 | - |
x * 36000, recombinant processed LOX, SDS-PAGE, x * 51000, recombinant processed LOXL1, SDS-PAGE, x * 29000, recombinant processed LOXL2, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme forms are differently involved in tumor suppression, cellular senescence, and chemotaxis | ? | - |
? | |
peptidyl-L-lysyl-peptide + O2 + H2O | Homo sapiens | substrates are proteins of the extracellular matrix | peptidyl-allysyl-peptide + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
enzyme forms: lysyl oxidase and 4 LOX-like proteins LOXL1, LOXL2, LOXL3, and LOXL4 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | LOX, LOXL1, LOXL2, LOXL3, and LOXL4, enzymes contain a conserved BMP-1 cleavage site at Gly134-Asp135 and Ser337-Asp338 | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant, N-terminally His6-tagged proteolytically processed forms of LOX, LOXL1, and LOXL2 from Escherichia coli inclusion bodies by nickel chelating affinity chromatography, to over 95% purity | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
convertion of recombinant, N-terminally His6-tagged proteolytically processed forms of LOX, LOXL1, and LOXL2 from Escherichia coli inclusion bodies to active enzymes by denaturation with 8 M urea prior to stepwise dialysis in presence of 2% N-laurylsarcosinate and 0.2 mM CuCl2 | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.054 | - |
recombinant His-tagged LOXL1, substrate benzylamine | Homo sapiens |
0.097 | - |
recombinant His-tagged LOX, substrate benzylamine | Homo sapiens |
0.15 | - |
recombinant His-tagged LOXL2, substrate benzylamine | Homo sapiens |
Storage Stability | Organism |
---|---|
lyophilized purified recombinant LOX, loss of 73% activity without stabilizing agent, loss of 4% activity in presence of 10 mM trehalose | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzylamine + O2 + H2O | - |
Homo sapiens | benzaldehyde + NH3 + H2O2 | - |
? | |
additional information | the enzyme forms are differently involved in tumor suppression, cellular senescence, and chemotaxis | Homo sapiens | ? | - |
? | |
additional information | lysyl oxidase and 4 LOX-like proteins LOXL, LOXL2, LOXL3, and LOXL4 all contain a cytokine receptor-like domain | Homo sapiens | ? | - |
? | |
peptidyl-L-lysyl-peptide + O2 + H2O | substrates are proteins of the extracellular matrix | Homo sapiens | peptidyl-allysyl-peptide + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36000, recombinant processed LOX, SDS-PAGE, x * 51000, recombinant processed LOXL1, SDS-PAGE, x * 29000, recombinant processed LOXL2, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
LOX | - |
Homo sapiens |
LOXL | - |
Homo sapiens |
lysyl oxidase | - |
Homo sapiens |
lysyl oxidase-like protein | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
lysyl-tyrosyl-quinone | LOX, LOXL1, LOXL2, LOXL3, and LOXL4 contain binding residues | Homo sapiens |