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Literature summary for 1.4.3.13 extracted from

  • Harris, E.D.; Gonnerman, W.A.; Savage, J.E.; O'Dell, B.L.
    Connective tissue amine oxidase. II. Purification and partial characterization of lysyl oxidase from chick aorta (1974), Biochim. Biophys. Acta, 341, 332-344.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
pyridoxal pyridoxal phosphate or closely related compound Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol weakly inhibitory Gallus gallus
beta-aminopropionitrile total inhibition at 1 mM Gallus gallus
diethyldithiocarbamate
-
Gallus gallus
dithiothreitol
-
Gallus gallus
hydroxylamine
-
Gallus gallus
phenylhydrazine
-
Gallus gallus
Semicarbazide
-
Gallus gallus
Zn2+ slightly inhibitory Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ copper metalloenzyme contains 0.14% copper, important for enzymatic function Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
x * 59000, x * 61000, SDS-PAGE, may be isoenzymes or heterologous subunits Gallus gallus
61000
-
x * 59000, x * 61000, SDS-PAGE, may be isoenzymes or heterologous subunits Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
embryo
-

Purification (Commentary)

Purification (Comment) Organism
affinity, ion-exchange Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
aorta
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
46390 cpm/mg Gallus gallus

Storage Stability

Storage Stability Organism
-15°C, powdered tissue, full enzyme activity for at least 4 months Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
peptidyl-L-hydroxylysyl-peptide + O2 + H2O
-
Gallus gallus peptidyl-L-2-amino-5-hydroxy-6-oxohexanoyl-peptide + NH3 + H2O2
-
?
peptidyl-L-lysyl-peptide + O2 + H2O part of elastin and collagen Gallus gallus peptidyl-L-allysyl-peptide + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 59000, x * 61000, SDS-PAGE, may be isoenzymes or heterologous subunits Gallus gallus

Synonyms

Synonyms Comment Organism
lysyl oxidase amine oxidase, oxidative deamination Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
assay at Gallus gallus