Literature summary for 1.4.3.12 extracted from

Tokieda, T.; Niimura, T.; Takamura,F.; Yamaha, T.
Purification and some properties of cyclohexylamine oxidase from a Pseudomonas sp. (1977), J. Biochem., 81, 851-858.

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Inhibitors

Inhibitors	Comment	Organism	Structure
Cd2+	38% inhibition at 10 mM	Pseudomonas sp.
Cu2+	complete inhibition at 10 mM	Pseudomonas sp.
Hg2+	complete inhibition at 10 mM	Pseudomonas sp.
N-ethylmaleimide	27% inhibition at 0.05 mM	Pseudomonas sp.
p-chloromercuribenzoate	91% inhibition at 0.0005 mM, partially reversed by glutathione	Pseudomonas sp.
Quinacrine	45% inhibition at 2 mM, partially released by FAD	Pseudomonas sp.
Zn2+	48% inhibition at 10 mM	Pseudomonas sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.25	-	Cyclohexylamine	-	Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
80000	-	gel filtration	Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cyclohexylamine + O2 + H2O	Pseudomonas sp.	highly specific for alicyclic primary amines, oxygen is the only electron acceptor	cyclohexanone + NH3 + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp.	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
to homogeneity, fractionation and chromatography steps	Pseudomonas sp.

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell culture	-	Pseudomonas sp.	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
29.6	-	-	Pseudomonas sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1,2,3,4-tetrahydro-2-naphthylamine + O2 + H2O	23% of the activity compared to cyclohexylamine	Pseudomonas sp.	? + NH3 + H2O2	-	?
1,2-cyclohexanediamine + O2 + H2O	18% of the activity compared to cyclohexylamine	Pseudomonas sp.	cyclohexaneamine + NH3 + H2O2	-	?
cycloheptylamine + O2 + H2O	42% of the activity compared to cyclohexylamine	Pseudomonas sp.	cycloheptanone + NH3 + H2O2	-	?
cyclohexylamine + O2 + H2O	highly specific for alicyclic primary amines, oxygen is the only electron acceptor	Pseudomonas sp.	cyclohexanone + NH3 + H2O2	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
35	-	-	Pseudomonas sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.8	-	-	Pseudomonas sp.

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Pseudomonas sp.

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Release 2023.1 (January 2023)