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Literature summary for 1.4.3.10 extracted from
- Specific measurement of putrescine with putrescine oxidase and aminobutyraldehyde dehydrogenase (1988), Agric. Biol. Chem., 52, 2865-2871.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | specific measurement of putrescine with putrescine oxidase and aminobutyraldehyde dehydrogenase | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| putrescine | substrate inhibition at 10 mM or above | Pseudomonas putida |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
putrescine | - |
Pseudomonas putida | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas putida |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in presence of 25% glycerol, 1 year, stable | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cadaverine + O2 + H2O | at 11% of the activity with putrescine | Pseudomonas putida | 5-aminopentanal + NH3 + H2O2 | - |
? | |
| putrescine + O2 + H2O | - |
Pseudomonas putida | 4-aminobutanal + NH3 + H2O2 | - |
? | |
| spermidine + O2 + H2O | at 9% of the activity with putrescine | Pseudomonas putida | 4-aminobutyraldehyde + 1,3-diaminopropane + H2O2 | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
pH 7.5, 20 min, 17% loss of activity | Pseudomonas putida |
| 50 | - |
pH 7.5, 20 min, 62% loss of activity | Pseudomonas putida |
| 60 | - |
pH 7.5, 20 min, 93% loss of activity | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 11.7 | - |
Pseudomonas putida |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10.5 | stable | Pseudomonas putida |
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