Protein Variants | Comment | Organism |
---|---|---|
D203A | dual specificity for NAD+ and NADP+ | Thermoactinomyces intermedius |
D203A/I204R | high affinity for NADP+ | Thermoactinomyces intermedius |
D203A/I204R/D210R | high affinity for NADP+ | Thermoactinomyces intermedius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
L-Ile | - |
Thermoactinomyces intermedius | |
2.5 | - |
NADP+ | mutant D203A/I204R/D210R | Thermoactinomyces intermedius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoactinomyces intermedius | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ile + H2O + NAD+ | - |
Thermoactinomyces intermedius | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
? | |
L-Leu + H2O + NAD+ | - |
Thermoactinomyces intermedius | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-leucine + H2O + NADP+ | - |
Thermoactinomyces intermedius | 4-methyl-2-oxopentanoate + NH3 + NADPH + H+ | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
NADP+ | mutant enzyme D203A/I204R and D203A/I204R/D210R | Thermoactinomyces intermedius | |
90 | - |
NAD+ | wild-type enzyme | Thermoactinomyces intermedius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermoactinomyces intermedius | |
NADH | - |
Thermoactinomyces intermedius | |
NADP+ | wild-type enzyme is inactive, mutant enzyme D203A exhibits dual specificity for NAD+ and NADP+, mutant enzymes D203A/I204R and D203A/I204R/D210R show high affinity for NADP+ | Thermoactinomyces intermedius |