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Literature summary for 1.4.1.8 extracted from

  • Hyun, C.G.; Kim, S.S.; Suh, J.W.
    Identification of essential amino acid residues in valine dehydrogenase from Streptomyces albus (2006), J. Microbiol., 44, 50-53.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C251S kcat for deamination is 13% of the wild-type value Streptomyces albus
C30S kcat for deamination is 13% of the wild-type value Streptomyces albus
V305L kcat for deamination is 0.1% of the wild-type value Streptomyces albus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.091
-
NAD+ pH 10.5, mutant enzyme C30S Streptomyces albus
0.15
-
NAD+ pH 10.5, wild-type enzyme Streptomyces albus
0.159
-
NAD+ pH 10.5, mutant enzyme C251S Streptomyces albus
0.37
-
NAD+ pH 10.5, mutant enzyme V305L Streptomyces albus
0.8
-
L-valine pH 10.5, mutant enzyme C30S Streptomyces albus
1.6
-
L-valine pH 10.5, mutant enzyme C251S Streptomyces albus
1.686
-
L-valine pH 10.5, wild-type enzyme Streptomyces albus
100
-
L-valine pH 10.5, mutant enzyme V305L Streptomyces albus

Organism

Organism UniProt Comment Textmining
Streptomyces albus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces albus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoleucine + H2O + NAD+ 27.5% of the activity with L-valine Streptomyces albus 3-methyl-2-oxopentanoate + NADH + NH3
-
?
L-leucine + H2O + NAD+ 15% of the activity with L-valine Streptomyces albus 2-oxoisocaproate + NADH + NH3
-
?
L-norleucine + H2O + NAD+ 15% of the activity with L-valine Streptomyces albus 2-oxocaproate + NADH + NH3
-
?
L-norvaline + H2O + NAD+ as active as with L-valine Streptomyces albus 2-oxovalerate + NH3 + NADH
-
?
L-valine + NAD+ + H2O
-
Streptomyces albus 2-oxoisovalerate + NH3 + NADH
-
?

Synonyms

Synonyms Comment Organism
ValDH
-
Streptomyces albus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Streptomyces albus
NADH
-
Streptomyces albus