Protein Variants | Comment | Organism |
---|---|---|
C251S | kcat for deamination is 13% of the wild-type value | Streptomyces albus |
C30S | kcat for deamination is 13% of the wild-type value | Streptomyces albus |
V305L | kcat for deamination is 0.1% of the wild-type value | Streptomyces albus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.091 | - |
NAD+ | pH 10.5, mutant enzyme C30S | Streptomyces albus | |
0.15 | - |
NAD+ | pH 10.5, wild-type enzyme | Streptomyces albus | |
0.159 | - |
NAD+ | pH 10.5, mutant enzyme C251S | Streptomyces albus | |
0.37 | - |
NAD+ | pH 10.5, mutant enzyme V305L | Streptomyces albus | |
0.8 | - |
L-valine | pH 10.5, mutant enzyme C30S | Streptomyces albus | |
1.6 | - |
L-valine | pH 10.5, mutant enzyme C251S | Streptomyces albus | |
1.686 | - |
L-valine | pH 10.5, wild-type enzyme | Streptomyces albus | |
100 | - |
L-valine | pH 10.5, mutant enzyme V305L | Streptomyces albus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces albus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces albus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + H2O + NAD+ | 27.5% of the activity with L-valine | Streptomyces albus | 3-methyl-2-oxopentanoate + NADH + NH3 | - |
? | |
L-leucine + H2O + NAD+ | 15% of the activity with L-valine | Streptomyces albus | 2-oxoisocaproate + NADH + NH3 | - |
? | |
L-norleucine + H2O + NAD+ | 15% of the activity with L-valine | Streptomyces albus | 2-oxocaproate + NADH + NH3 | - |
? | |
L-norvaline + H2O + NAD+ | as active as with L-valine | Streptomyces albus | 2-oxovalerate + NH3 + NADH | - |
? | |
L-valine + NAD+ + H2O | - |
Streptomyces albus | 2-oxoisovalerate + NH3 + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ValDH | - |
Streptomyces albus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Streptomyces albus | |
NADH | - |
Streptomyces albus |