Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
particle-bound | leaves, seedling shoots | Phaseolus vulgaris | - |
- |
particle-bound | leaves, seedling shoots | Triticum aestivum | - |
- |
particle-bound | leaves, seedling shoots | Pisum sativum | - |
- |
particle-bound | leaves, seedling shoots | Zea mays | - |
- |
particle-bound | leaves, seedling shoots | Glycine max | - |
- |
particle-bound | leaves, seedling shoots | Plantago sp. | - |
- |
soluble | roots | Phaseolus vulgaris | - |
- |
soluble | roots | Pisum sativum | - |
- |
soluble | roots | Glycine max | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-valine + NAD(P)+ + H2O | Pisum sativum | two forms of enzyme activity: enzyme associated with subcellular structures in shoots or leaves probably plays a biosynthetic role for valine production and the soluble enzyme in roots plays a degradative role in valine catabolism | 2-oxoisovalerate + NH3 + NAD(P)H | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Phaseolus vulgaris | - |
- |
- |
Pisum sativum | - |
- |
- |
Plantago sp. | - |
- |
- |
Triticum aestivum | - |
- |
- |
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
soluble enzyme from roots, 3.9fold purification | Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | subcellular particles | Triticum aestivum | - |
leaf | subcellular particles | Zea mays | - |
leaf | subcellular particles | Plantago sp. | - |
additional information | enzyme activity observed in seedling shoots, but not in roots | Triticum aestivum | - |
additional information | enzyme activity observed in seedling shoots, but not in roots | Zea mays | - |
root | soluble enzyme | Phaseolus vulgaris | - |
root | soluble enzyme | Pisum sativum | - |
root | soluble enzyme | Glycine max | - |
seedling | enzyme associated with subcellular particles | Phaseolus vulgaris | - |
seedling | enzyme associated with subcellular particles | Triticum aestivum | - |
seedling | enzyme associated with subcellular particles | Pisum sativum | - |
seedling | enzyme associated with subcellular particles | Zea mays | - |
seedling | enzyme associated with subcellular particles | Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
values for reductive amination at pH 8.5 | Phaseolus vulgaris |
additional information | - |
values for reductive amination at pH 8.5 | Triticum aestivum |
additional information | - |
values for reductive amination at pH 8.5 | Pisum sativum |
additional information | - |
values for reductive amination at pH 8.5 | Zea mays |
additional information | - |
values for reductive amination at pH 8.5 | Glycine max |
additional information | - |
values for reductive amination at pH 8.5 | Plantago sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-valine + NAD(P)+ + H2O | NADP+ | Phaseolus vulgaris | 2-oxoisovalerate + NH3 + NAD(P)H | NADPH | r | |
L-valine + NAD(P)+ + H2O | NADP+ | Triticum aestivum | 2-oxoisovalerate + NH3 + NAD(P)H | NADPH | r | |
L-valine + NAD(P)+ + H2O | NADP+ | Pisum sativum | 2-oxoisovalerate + NH3 + NAD(P)H | NADPH | r | |
L-valine + NAD(P)+ + H2O | NADP+ | Zea mays | 2-oxoisovalerate + NH3 + NAD(P)H | NADPH | r | |
L-valine + NAD(P)+ + H2O | NADP+ | Glycine max | 2-oxoisovalerate + NH3 + NAD(P)H | NADPH | r | |
L-valine + NAD(P)+ + H2O | NADP+ | Plantago sp. | 2-oxoisovalerate + NH3 + NAD(P)H | NADPH | r | |
L-valine + NAD(P)+ + H2O | NADP+ | Phaseolus vulgaris | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | NADP+ | Triticum aestivum | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | NADP+ | Pisum sativum | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | NADP+ | Zea mays | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | NADP+ | Glycine max | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | NADP+ | Plantago sp. | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | soluble enzyme in roots: ratio of rate of reductive amination of valine ketoanalogue to the rate of oxidative deamination of L-valine is 1:1 | Pisum sativum | 2-oxoisovalerate + NH3 + NAD(P)H | NADPH | r | |
L-valine + NAD(P)+ + H2O | soluble enzyme in roots: ratio of rate of reductive amination of valine ketoanalogue to the rate of oxidative deamination of L-valine is 1:1 | Pisum sativum | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | in the enzyme associated with subcellular structures in shoots it is 2.5:1 | Phaseolus vulgaris | 2-oxoisovalerate + NH3 + NAD(P)H | NADPH | r | |
L-valine + NAD(P)+ + H2O | in the enzyme associated with subcellular structures in shoots it is 2.5:1 | Triticum aestivum | 2-oxoisovalerate + NH3 + NAD(P)H | NADPH | r | |
L-valine + NAD(P)+ + H2O | in the enzyme associated with subcellular structures in shoots it is 2.5:1 | Pisum sativum | 2-oxoisovalerate + NH3 + NAD(P)H | NADPH | r | |
L-valine + NAD(P)+ + H2O | in the enzyme associated with subcellular structures in shoots it is 2.5:1 | Zea mays | 2-oxoisovalerate + NH3 + NAD(P)H | NADPH | r | |
L-valine + NAD(P)+ + H2O | in the enzyme associated with subcellular structures in shoots it is 2.5:1 | Glycine max | 2-oxoisovalerate + NH3 + NAD(P)H | NADPH | r | |
L-valine + NAD(P)+ + H2O | in the enzyme associated with subcellular structures in shoots it is 2.5:1 | Plantago sp. | 2-oxoisovalerate + NH3 + NAD(P)H | NADPH | r | |
L-valine + NAD(P)+ + H2O | in the enzyme associated with subcellular structures in shoots it is 2.5:1 | Phaseolus vulgaris | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | in the enzyme associated with subcellular structures in shoots it is 2.5:1 | Triticum aestivum | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | in the enzyme associated with subcellular structures in shoots it is 2.5:1 | Pisum sativum | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | in the enzyme associated with subcellular structures in shoots it is 2.5:1 | Zea mays | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | in the enzyme associated with subcellular structures in shoots it is 2.5:1 | Glycine max | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | in the enzyme associated with subcellular structures in shoots it is 2.5:1 | Plantago sp. | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | two forms of enzyme activity: enzyme associated with subcellular structures in shoots or leaves probably plays a biosynthetic role for valine production and the soluble enzyme in roots plays a degradative role in valine catabolism | Pisum sativum | 2-oxoisovalerate + NH3 + NAD(P)H | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | 8.6 | shoots, reductive amination of valine ketoanalogue | Pisum sativum |
9.3 | - |
roots, reductive amination of valine ketoanalogue | Pisum sativum |
9.4 | 9.6 | shoots, oxidative deamination of L-valine | Pisum sativum |
11 | - |
roots, oxidative deamination of L-valine | Pisum sativum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | plant enzyme not coupled to NAD+ and NADH | Phaseolus vulgaris | |
additional information | plant enzyme not coupled to NAD+ and NADH | Triticum aestivum | |
additional information | plant enzyme not coupled to NAD+ and NADH | Pisum sativum | |
additional information | plant enzyme not coupled to NAD+ and NADH | Zea mays | |
additional information | plant enzyme not coupled to NAD+ and NADH | Glycine max | |
additional information | plant enzyme not coupled to NAD+ and NADH | Plantago sp. | |
NADP+ | in plants enzyme is coupled exclusively to NADP+ | Phaseolus vulgaris | |
NADP+ | in plants enzyme is coupled exclusively to NADP+ | Triticum aestivum | |
NADP+ | in plants enzyme is coupled exclusively to NADP+ | Pisum sativum | |
NADP+ | in plants enzyme is coupled exclusively to NADP+ | Zea mays | |
NADP+ | in plants enzyme is coupled exclusively to NADP+ | Glycine max | |
NADP+ | in plants enzyme is coupled exclusively to NADP+ | Plantago sp. | |
NADPH | in plants valine dehydrogenase coupled exclusively to NADPH | Phaseolus vulgaris | |
NADPH | in plants valine dehydrogenase coupled exclusively to NADPH | Triticum aestivum | |
NADPH | in plants valine dehydrogenase coupled exclusively to NADPH | Pisum sativum | |
NADPH | in plants valine dehydrogenase coupled exclusively to NADPH | Zea mays | |
NADPH | in plants valine dehydrogenase coupled exclusively to NADPH | Glycine max | |
NADPH | in plants valine dehydrogenase coupled exclusively to NADPH | Plantago sp. |