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Literature summary for 1.4.1.4 extracted from

  • Prakash, P.; Walvekar, A.; Punekar, N.; Bhaumik, P.
    Purification, crystallization and preliminary X-ray diffraction analysis of NADP-dependent glutamate dehydrogenase from Aspergillus niger (2014), Acta Crystallogr. Sect. F, 70, 1508-1512 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aspergillus niger

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.9 A resolution, space group R32, with unit-cell parameters a = b = 173.8, c = 241.5 A, alpha = beta = 90°, gamma = 120°. The crystals exhibit a high solvent content (83.0%) with only one molecule in the asymmetric unit Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
631
-
 pH 8.0, 23°C Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NADP+
-
 Aspergillus niger 2-oxoglutarate + NH3 + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 49390, calculated, x * 49400, DS-PAGE Aspergillus niger