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Literature summary for 1.4.1.4 extracted from
- Extremely thermostable glutamate dehydrogenase (GDH) from the freshwater archaeon Thermococcus waiotapuensis: cloning and comparison with two marine hyperthermophilic GDHs (2002), Extremophiles, 6, 151-159.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Thermococcus waiotapuensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCl | 1 M, 30% loss of activity | Thermococcus waiotapuensis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | 1 | NADP+ | pH 8.0, 80°C | Thermococcus waiotapuensis | |
| 0.17 | - |
NADPH | pH 8.0, 80°C | Thermococcus waiotapuensis | |
| 0.27 | - |
2-oxoglutarate | pH 8.0, 80°C | Thermococcus waiotapuensis | |
| 1.3 | - |
L-glutamate | pH 8.0, 80°C | Thermococcus waiotapuensis | |
| 9.2 | - |
NH3 | pH 8.0, 80°C | Thermococcus waiotapuensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 10 mM, 1.3fold increase in activity | Thermococcus waiotapuensis | |
| MgSO4 | 10 mM, 1.3fold increase in activity | Thermococcus waiotapuensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
6 * 45000, SDS-PAGE | Thermococcus waiotapuensis |
| 259000 | - |
gel filtration | Thermococcus waiotapuensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus waiotapuensis | Q977X9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermococcus waiotapuensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 160 | - |
pH 8.0, 80°C | Thermococcus waiotapuensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + H2O + NADP+ | - |
Thermococcus waiotapuensis | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 45000, SDS-PAGE | Thermococcus waiotapuensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
assay at | Thermococcus waiotapuensis |
| 84 | - |
- |
Thermococcus waiotapuensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
some decrease in specific activity is above 90°C | Thermococcus waiotapuensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 104 | - |
half-life is 4.2 min in absence of KCl and 3.3 min in presence of 1 M KCl | Thermococcus waiotapuensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 141 | - |
L-glutamate | pH 8.0, 80°C | Thermococcus waiotapuensis | |
| 163 | - |
NH3 | pH 8.0, 80°C | Thermococcus waiotapuensis | |
| 1209 | - |
NADP+ | pH 8.0, 80°C | Thermococcus waiotapuensis | |
| 2530 | - |
2-oxoglutarate | pH 8.0, 80°C | Thermococcus waiotapuensis | |
| 14710 | - |
NADPH | pH 8.0, 80°C | Thermococcus waiotapuensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Thermococcus waiotapuensis |
| 8 | - |
assay at | Thermococcus waiotapuensis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 133 | - |
NADP+ | pH 8.0, 80°C | Thermococcus waiotapuensis | |
| 183 | - |
L-glutamate | pH 8.0, 80°C | Thermococcus waiotapuensis | |
| 683 | - |
2-oxoglutarate | pH 8.0, 80°C | Thermococcus waiotapuensis | |
| 1500 | - |
NH3 | pH 8.0, 80°C | Thermococcus waiotapuensis | |
| 2500 | - |
NADPH | pH 8.0, 80°C | Thermococcus waiotapuensis | |
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