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Literature summary for 1.4.1.4 extracted from

  • Sakamoto, N.; Kotre, A.M.; Savageau, M.A.
    Glutamate dehydrogenase from Escherichia coli: purification and properties (1975), J. Bacteriol., 124, 775-783.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
inactivated by freezing/thawing Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
guanidine hydrochloride complete loss of activity Escherichia coli
L-homoserine competitive inhibitor with respect to both ammonia and glutamine Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
NADPH reductive amination Escherichia coli
0.042
-
NADP+ oxidative deamination Escherichia coli
0.64
-
2-oxoglutarate reductive amination Escherichia coli
1.1
-
NH4+ reductive amination Escherichia coli
1.3
-
L-glutamate oxidative deamination Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
alpha6, 6 * 50000, SDS-PAGE Escherichia coli
300000
-
sedimentation equilibrium Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + NADP+ + H2O Escherichia coli
-
2-oxoglutarate + NADPH + NH3
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
180
-
oxidative deamination at 25°C Escherichia coli

Storage Stability

Storage Stability Organism
4°C, water, 20 mM potassium phosphate or sodium phosphate, pH 7.2, at least 3 months Escherichia coli
room temperature, several months stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH4+ + NADPH
-
Escherichia coli L-glutamate + NADP+
-
?
L-glutamate + NADP+ + H2O
-
Escherichia coli 2-oxoglutarate + NADPH + NH3
-
r

Subunits

Subunits Comment Organism
homohexamer alpha6, 6 * 50000, SDS-PAGE Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
inactivated by freezing Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Escherichia coli
NADPH
-
Escherichia coli