Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | slight activation up to 1 mM | Escherichia coli | |
ATP | at 2 mM, 50% activation | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | inhibitory at higher concentrations than 1 mM | Escherichia coli | |
Urea | at 8 M stable for 10 min | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46000, SDS-PAGE | Escherichia coli |
245000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Escherichia coli | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | Escherichia coli MRE 600 | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli MRE 600 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
250 | - |
oxidative deamination | Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, 0.1 M phosphate buffer, several days, no loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | - |
Escherichia coli | 2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | - |
Escherichia coli MRE 600 | 2-oxoglutarate + NADPH + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
reductive amination | Escherichia coli |
9 | - |
oxidative deamination | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli | |
NADPH | - |
Escherichia coli |