Activating Compound | Comment | Organism | Structure |
---|---|---|---|
KCl | up to 50mM | Trypanosoma cruzi | |
NaCl | up to 50 mM | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | more than 50 mM | Trypanosoma cruzi | |
NaCl | more than 50 mM | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
NADPH | reductive amination | Trypanosoma cruzi | |
0.0064 | - |
NADP+ | oxidative deamination | Trypanosoma cruzi | |
1 | - |
2-oxoglutarate | reductive amination | Trypanosoma cruzi | |
1.7 | - |
NH4+ | reductive amination | Trypanosoma cruzi | |
2 | - |
L-glutamate | reductive amination | Trypanosoma cruzi | |
66 | - |
NH4+ | reductive amination | Trypanosoma cruzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
4 * 64000, SDS-PAGE | Trypanosoma cruzi |
280000 | - |
gel filtration | Trypanosoma cruzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Trypanosoma cruzi | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Trypanosoma cruzi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
51.2 | - |
reductive amination | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH4+ + NADPH | - |
Trypanosoma cruzi | L-glutamate + NADP+ | - |
? | |
L-glutamate + NADP+ + H2O | - |
Trypanosoma cruzi | 2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | - |
Trypanosoma cruzi | 2-oxoglutarate + NADPH + NH3 | biphasic kinetic behavior for ammonia | r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 64000, SDS-PAGE | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
oxidative deamination | Trypanosoma cruzi |
8 | - |
reductive amination | Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Trypanosoma cruzi | |
NADPH | - |
Trypanosoma cruzi |