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Literature summary for 1.4.1.4 extracted from
- Isolation and characterisation of glutamate dehydrogenase from Mycobacterium smegmatis CDC 46 (1980), Biochim. Biophys. Acta, 615, 299-308.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | activates up to 0.1 mM | Mycolicibacterium smegmatis |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | at 1 mM and 10 mM, 30% inhibition and 100% inhibition | Mycolicibacterium smegmatis | |
| Hg2+ | at 1 mM, 10% inhibition | Mycolicibacterium smegmatis | |
| Zn2+ | over 0.1 mM | Mycolicibacterium smegmatis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
alpha6, 6 * 40000, SDS-PAGE | Mycolicibacterium smegmatis |
| 245500 | - |
gel filtration | Mycolicibacterium smegmatis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + NADP+ + H2O | Mycolicibacterium smegmatis | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | Mycolicibacterium smegmatis CDC46 | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium smegmatis | - |
- |
- |
| Mycolicibacterium smegmatis CDC46 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycolicibacterium smegmatis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 80.2 | - |
reductive amination | Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + NADP+ + H2O | - |
Mycolicibacterium smegmatis | 2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | - |
Mycolicibacterium smegmatis CDC46 | 2-oxoglutarate + NADPH + NH3 | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | alpha6, 6 * 40000, SDS-PAGE | Mycolicibacterium smegmatis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
reductive amination and oxidative deamination | Mycolicibacterium smegmatis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
reductive amination | Mycolicibacterium smegmatis |
| 8.8 | 9.8 | oxidative deamination | Mycolicibacterium smegmatis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Mycolicibacterium smegmatis | |
| NADPH | - |
Mycolicibacterium smegmatis | |
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