Crystallization (Comment) | Organism |
---|---|
- |
Limosilactobacillus fermentum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.044 | - |
NADP+ | oxidative deamination | Limosilactobacillus fermentum | |
0.078 | - |
NADPH | reductive amination | Limosilactobacillus fermentum | |
5.6 | - |
2-oxoglutarate | reductive amination | Limosilactobacillus fermentum | |
6.76 | - |
NH4+ | reductive amination | Limosilactobacillus fermentum | |
79 | - |
L-glutamate | oxidative deamination | Limosilactobacillus fermentum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
alpha6, 6 * 50000, SDS-PAGE | Limosilactobacillus fermentum |
300000 | - |
gel filtration | Limosilactobacillus fermentum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Lactococcus lactis | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | Streptococcus equinus | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | Enterococcus faecalis | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | Limosilactobacillus fermentum | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | Streptococcus equinus IFO 12553 | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | Lactococcus lactis IFO 12546 | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | Enterococcus faecalis IFO 12964 | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | Limosilactobacillus fermentum IFO 3071 | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Enterococcus faecalis IFO 12964 | - |
- |
- |
Lactococcus lactis | - |
- |
- |
Lactococcus lactis IFO 12546 | - |
- |
- |
Limosilactobacillus fermentum | - |
- |
- |
Limosilactobacillus fermentum IFO 3071 | - |
- |
- |
Streptococcus equinus | - |
- |
- |
Streptococcus equinus IFO 12553 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Limosilactobacillus fermentum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
57.6 | - |
reductive amination | Limosilactobacillus fermentum |
Storage Stability | Organism |
---|---|
-20°C, potassium phosphate buffer, pH 7.4, containing 0.01% 2-mercaptoethanol and 10% glycerol, 100% activity | Limosilactobacillus fermentum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH4+ + NADPH | - |
Limosilactobacillus fermentum | L-glutamate + NADP+ | - |
? | |
2-oxoglutarate + NH4+ + NADPH | - |
Limosilactobacillus fermentum IFO 3071 | L-glutamate + NADP+ | - |
? | |
L-glutamate + NADP+ + H2O | - |
Lactococcus lactis | 2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | - |
Streptococcus equinus | 2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | - |
Enterococcus faecalis | 2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | - |
Limosilactobacillus fermentum | 2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | - |
Streptococcus equinus IFO 12553 | 2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | - |
Lactococcus lactis IFO 12546 | 2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | - |
Enterococcus faecalis IFO 12964 | 2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | - |
Limosilactobacillus fermentum IFO 3071 | 2-oxoglutarate + NADPH + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | alpha6, 6 * 50000, SDS-PAGE | Limosilactobacillus fermentum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
after 10 min in phosphate buffer pH 7.4 100% activity | Limosilactobacillus fermentum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
reductive amination | Limosilactobacillus fermentum |
9 | - |
oxidative deamination | Limosilactobacillus fermentum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Lactococcus lactis | |
NADP+ | - |
Streptococcus equinus | |
NADP+ | - |
Enterococcus faecalis | |
NADP+ | - |
Limosilactobacillus fermentum | |
NADPH | - |
Lactococcus lactis | |
NADPH | - |
Streptococcus equinus | |
NADPH | - |
Enterococcus faecalis | |
NADPH | - |
Limosilactobacillus fermentum |