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Literature summary for 1.4.1.4 extracted from
- Purification, crystallization and properties of NADP+-specific glutamate dehydrogenase from Lactobacillus fermentum (1985), Agric. Biol. Chem., 49, 117-123.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Limosilactobacillus fermentum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.044 | - |
NADP+ | oxidative deamination | Limosilactobacillus fermentum |  |
| 0.078 | - |
NADPH | reductive amination | Limosilactobacillus fermentum | |
| 5.6 | - |
2-oxoglutarate | reductive amination | Limosilactobacillus fermentum | |
| 6.76 | - |
NH4+ | reductive amination | Limosilactobacillus fermentum | |
| 79 | - |
L-glutamate | oxidative deamination | Limosilactobacillus fermentum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
alpha6, 6 * 50000, SDS-PAGE | Limosilactobacillus fermentum |
| 300000 | - |
gel filtration | Limosilactobacillus fermentum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + NADP+ + H2O | Lactococcus lactis | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | Streptococcus equinus | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | Enterococcus faecalis | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | Limosilactobacillus fermentum | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | Streptococcus equinus IFO 12553 | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | Lactococcus lactis IFO 12546 | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | Enterococcus faecalis IFO 12964 | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | Limosilactobacillus fermentum IFO 3071 | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecalis | - |
- |
- |
| Enterococcus faecalis IFO 12964 | - |
- |
- |
| Lactococcus lactis | - |
- |
- |
| Lactococcus lactis IFO 12546 | - |
- |
- |
| Limosilactobacillus fermentum | - |
- |
- |
| Limosilactobacillus fermentum IFO 3071 | - |
- |
- |
| Streptococcus equinus | - |
- |
- |
| Streptococcus equinus IFO 12553 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Limosilactobacillus fermentum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 57.6 | - |
reductive amination | Limosilactobacillus fermentum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, potassium phosphate buffer, pH 7.4, containing 0.01% 2-mercaptoethanol and 10% glycerol, 100% activity | Limosilactobacillus fermentum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + NH4+ + NADPH | - |
Limosilactobacillus fermentum | L-glutamate + NADP+ | - |
? | |
| 2-oxoglutarate + NH4+ + NADPH | - |
Limosilactobacillus fermentum IFO 3071 | L-glutamate + NADP+ | - |
? | |
| L-glutamate + NADP+ + H2O | - |
Lactococcus lactis | 2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | - |
Streptococcus equinus | 2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | - |
Enterococcus faecalis | 2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | - |
Limosilactobacillus fermentum | 2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | - |
Streptococcus equinus IFO 12553 | 2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | - |
Lactococcus lactis IFO 12546 | 2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | - |
Enterococcus faecalis IFO 12964 | 2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | - |
Limosilactobacillus fermentum IFO 3071 | 2-oxoglutarate + NADPH + NH3 | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | alpha6, 6 * 50000, SDS-PAGE | Limosilactobacillus fermentum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
after 10 min in phosphate buffer pH 7.4 100% activity | Limosilactobacillus fermentum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
reductive amination | Limosilactobacillus fermentum |
| 9 | - |
oxidative deamination | Limosilactobacillus fermentum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Lactococcus lactis | |
| NADP+ | - |
Streptococcus equinus | |
| NADP+ | - |
Enterococcus faecalis | |
| NADP+ | - |
Limosilactobacillus fermentum | |
| NADPH | - |
Lactococcus lactis | |
| NADPH | - |
Streptococcus equinus | |
| NADPH | - |
Enterococcus faecalis | |
| NADPH | - |
Limosilactobacillus fermentum | |
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