Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | at 0.01 mM, 35% inhibition | Streptomyces fradiae | |
ADP | at 1 mM, 57% inhibition for oxidative deamination and 23% inhibition for reductive amination respectively | Streptomyces fradiae | |
AMP | slight inhibitory | Streptomyces fradiae | |
HgCl2 | at 0.01 mM, 27% inhibition | Streptomyces fradiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
NADPH | reductive amination | Streptomyces fradiae | |
0.12 | - |
NADP+ | oxidative deamination | Streptomyces fradiae | |
1.54 | - |
2-oxoglutarate | reductive amination | Streptomyces fradiae | |
28.6 | - |
L-glutamate | oxidative deamination | Streptomyces fradiae | |
30.8 | - |
NH4+ | reductive amination | Streptomyces fradiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
alpha4, 4 * 49000, SDS-PAGE | Streptomyces fradiae |
200000 | - |
gel filtration | Streptomyces fradiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Streptomyces fradiae | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces fradiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces fradiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.68 | - |
oxidative deamination | Streptomyces fradiae |
10.92 | - |
reductive amination | Streptomyces fradiae |
Storage Stability | Organism |
---|---|
4°C and -25°C, 0.2 M Tris/HCl buffer, pH 7.4, 24 h or 2 months respectively, 100% activity | Streptomyces fradiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH4+ + NADPH | - |
Streptomyces fradiae | L-glutamate + NADP+ | - |
? | |
L-glutamate + NADP+ + H2O | - |
Streptomyces fradiae | 2-oxoglutarate + NADPH + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | alpha4, 4 * 49000, SDS-PAGE | Streptomyces fradiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
reductive amination | Streptomyces fradiae |
9.3 | - |
oxidative deamination | Streptomyces fradiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Streptomyces fradiae | |
NADPH | - |
Streptomyces fradiae |