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Literature summary for 1.4.1.4 extracted from

  • Vancurova, I.; Vancura, A.; Volc, J.; Kopecky, J.; Neuzil, J.; Basarova, G.; Behal, V.
    Purification and properties of NADP-dependent glutamate dehydrogenase from Streptomyces fradiae (1989), J. Gen. Microbiol., 135, 3311-3318.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate at 0.01 mM, 35% inhibition Streptomyces fradiae
ADP at 1 mM, 57% inhibition for oxidative deamination and 23% inhibition for reductive amination respectively Streptomyces fradiae
AMP slight inhibitory Streptomyces fradiae
HgCl2 at 0.01 mM, 27% inhibition Streptomyces fradiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
NADPH reductive amination Streptomyces fradiae
0.12
-
NADP+ oxidative deamination Streptomyces fradiae
1.54
-
2-oxoglutarate reductive amination Streptomyces fradiae
28.6
-
L-glutamate oxidative deamination Streptomyces fradiae
30.8
-
NH4+ reductive amination Streptomyces fradiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
alpha4, 4 * 49000, SDS-PAGE Streptomyces fradiae
200000
-
gel filtration Streptomyces fradiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + NADP+ + H2O Streptomyces fradiae
-
2-oxoglutarate + NADPH + NH3
-
r

Organism

Organism UniProt Comment Textmining
Streptomyces fradiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces fradiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.68
-
oxidative deamination Streptomyces fradiae
10.92
-
reductive amination Streptomyces fradiae

Storage Stability

Storage Stability Organism
4°C and -25°C, 0.2 M Tris/HCl buffer, pH 7.4, 24 h or 2 months respectively, 100% activity Streptomyces fradiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH4+ + NADPH
-
Streptomyces fradiae L-glutamate + NADP+
-
?
L-glutamate + NADP+ + H2O
-
Streptomyces fradiae 2-oxoglutarate + NADPH + NH3
-
r

Subunits

Subunits Comment Organism
homotetramer alpha4, 4 * 49000, SDS-PAGE Streptomyces fradiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
reductive amination Streptomyces fradiae
9.3
-
oxidative deamination Streptomyces fradiae

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Streptomyces fradiae
NADPH
-
Streptomyces fradiae