Activating Compound | Comment | Organism | Structure |
---|---|---|---|
KCl | at concentrations below 1 mM | Pyrococcus woesei | |
KCl | at concentrations below 2 mM | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AlCl3 | at 1 mM, 30% inhibition | Pyrococcus furiosus | |
AlCl3 | at 1 mM, 21% inhibition | Pyrococcus woesei | |
HgCl2 | at 1 mM, 64% inhibition | Pyrococcus furiosus | |
HgCl2 | at 1 mM, 45% inhibition | Pyrococcus woesei | |
MnCl2 | at 1 mM, 63% inhibition | Pyrococcus furiosus | |
MnCl2 | at 1 mM, 20% inhibition | Pyrococcus woesei | |
Pb(CH3COO)2 | at 1 mM, 59% inhibition | Pyrococcus furiosus | |
Pb(CH3COO)2 | at 1 mM, 64% inhibition | Pyrococcus woesei | |
pyridoxal 5'-phosphate | at 1 mM, 35% inhibition | Pyrococcus furiosus | |
pyridoxal 5'-phosphate | at 1 mM, 45% inhibition | Pyrococcus woesei | |
Urea | inactivation with 2 mM urea | Pyrococcus furiosus | |
Urea | fully active at 20°C in 8 mM urea | Pyrococcus woesei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
alpha6, 6 * 47000, SDS-PAGE | Pyrococcus furiosus |
47000 | - |
alpha6, 6 * 47000, SDS-PAGE | Pyrococcus woesei |
300000 | - |
gel filtration | Pyrococcus furiosus |
300000 | - |
gel filtration | Pyrococcus woesei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Pyrococcus furiosus | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | Pyrococcus woesei | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
strain DSM 3638 | - |
Pyrococcus woesei | - |
strain DSM 3773 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.3 | - |
oxidative deamination at 50°C and pH 8.2 | Pyrococcus furiosus |
24.3 | - |
oxidative deamination at 50°C and pH 8.2 | Pyrococcus woesei |
Storage Stability | Organism |
---|---|
4°C, 10 mM potassium phosphate buffer, pH 7.2, 0.1 mM EDTA, 0.1 mM DTT, 5 months, no activity loss | Pyrococcus furiosus |
4°C, 10 mM potassium phosphate buffer, pH 7.2, 0.1 mM EDTA, 0.1 mM DTT, 5 months, no activity loss | Pyrococcus woesei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | - |
Pyrococcus furiosus | 2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | - |
Pyrococcus woesei | 2-oxoglutarate + NADPH + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | alpha6, 6 * 47000, SDS-PAGE | Pyrococcus furiosus |
homohexamer | alpha6, 6 * 47000, SDS-PAGE | Pyrococcus woesei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
oxidative deamination | Pyrococcus furiosus |
100 | - |
oxidative deamination | Pyrococcus woesei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
105 | - |
full activity after 10 min, 50% activity after 90 min | Pyrococcus furiosus |
105 | - |
full activity after 10 min, 50% activity after 90 min | Pyrococcus woesei |
115 | - |
inactivation after 10 min | Pyrococcus furiosus |
115 | - |
inactivation after 10 min | Pyrococcus woesei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
reductive amination at 50°C | Pyrococcus furiosus |
7.4 | - |
reductive amination at 50°C | Pyrococcus woesei |
8.2 | - |
oxidative deamination at 50°C | Pyrococcus furiosus |
8.2 | - |
oxidative deamination at 50°C | Pyrococcus woesei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | - |
Pyrococcus furiosus |
4 | 10 | - |
Pyrococcus woesei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Pyrococcus furiosus | |
NADP+ | - |
Pyrococcus woesei | |
NADPH | - |
Pyrococcus furiosus | |
NADPH | - |
Pyrococcus woesei |