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Literature summary for 1.4.1.4 extracted from

  • Baars, J.J.P.; Op den Camp, H.J.M.; van Hoek, A.H.A.M.; van der Drift, C.; Van Griensven, L.J.L.D.; Visser, J.; Vogels, G.D.
    Purification and characterization of NADP-dependent glutamate dehydrogenase from the commercial mushroom Agaricus bisporus (1995), Curr. Microbiol., 30, 211-217.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ADP at 4 mM slight inhibitory Agaricus bisporus
ATP at 4 mM slight inhibitory Agaricus bisporus
oxaloacetate at 20 mM slight inhibitory Agaricus bisporus
pyruvate at 10 mM slight inhibitory Agaricus bisporus
Urea at 0°C 1 h at 2 M 65% inhibition Agaricus bisporus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.074
-
NADPH reductive amination at 33°C Agaricus bisporus
0.117
-
NADP+ oxidative deamination at 33°C Agaricus bisporus
2.1
-
NH4+ reductive amination at 33°C Agaricus bisporus
3.2
-
2-oxoglutarate reductive amination at 33°C Agaricus bisporus
27
-
L-glutamate oxidative deamination at 33°C Agaricus bisporus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
alpha6, 6 * 48000, SDS-PAGE Agaricus bisporus
330000
-
gel filtration Agaricus bisporus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + NADP+ + H2O Agaricus bisporus
-
2-oxoglutarate + NADPH + NH3
-
r

Organism

Organism UniProt Comment Textmining
Agaricus bisporus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.2
-
reductive amination at 33°C Agaricus bisporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH4+ + NADPH
-
Agaricus bisporus L-glutamate + NADP+
-
?
L-glutamate + NADP+ + H2O
-
Agaricus bisporus 2-oxoglutarate + NADPH + NH3
-
r

Subunits

Subunits Comment Organism
homohexamer alpha6, 6 * 48000, SDS-PAGE Agaricus bisporus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
34
-
reductive amination Agaricus bisporus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
12% activity loss after 1 h Agaricus bisporus
37
-
72% activity loss after 1 h Agaricus bisporus
55
-
complete activity loss after 1 h Agaricus bisporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
reductive amination Agaricus bisporus
9
-
oxidative deamination Agaricus bisporus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Agaricus bisporus
NADPH
-
Agaricus bisporus