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Literature summary for 1.4.1.3 extracted from

  • Islam, M.M.; Nautiyal, M.; Wynn, R.M.; Mobley, J.A.; Chuang, D.T.; Hutson, S.M.
    The branched chain amino acid (BCAA) metabolon: Interaction of glutamate dehydrogenase with the mitochondrial branched chain aminotransferase (BCATm) (2010), J. Biol. Chem., 285, 265-276.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.3

Activating Compound

Activating Compound Comment Organism Structure
ADP ADP induces an allosteric conformational change in GDH1, leading to a 2fold enhanced oxidative deamination Rattus norvegicus
leucine enhances the oxidative deamination reaction of GDH1 Rattus norvegicus
PMP-BCATm pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm) accelerates the oxidative deamination reaction of GDH1in the presence of branched-chain amino acids (Leu, Ile, Val). Reductive amination reaction is not affected Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
GDP strong allosteric inhibitor of GDH1 leading to a 90% reduction of activity. Addition of increasing concentrations of pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm) leads to an increasing protection from GDP inhibition Rattus norvegicus
GTP
-
 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P10860
-
-

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Rattus norvegicus
-
kidney
-
 Rattus norvegicus
-
pancreas
-
 Rattus norvegicus
-
skeletal muscle
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + NAD+ + H2O
-
 Rattus norvegicus 2-oxoglutarate + NADH + NH3
-
 r

Subunits

Subunits Comment Organism
homohexamer method of determination not further specified Rattus norvegicus

Synonyms

Synonyms Comment Organism
GDH1
-
 Rattus norvegicus
glutamate dehydrogenase
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 reductive amination at Rattus norvegicus
8
-
 oxidative deamination at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+ GDH1 uses NAD+ or NADP+ as coenzyme Rattus norvegicus
NADP+ GDH1 uses NAD+ or NADP+ as coenzyme Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00005
-
 GTP GTP alone Rattus norvegicus
0.00156
-
 GTP molar ratio pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm)/GDH1: 1 Rattus norvegicus
0.00312
-
 GTP molar ratio pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm)/GDH1: 2 Rattus norvegicus
0.00795
-
 GTP molar ratio pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm)/GDH1: 6 Rattus norvegicus
0.00806
-
 GTP molar ratio pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm)/GDH1: 4 Rattus norvegicus

General Information

General Information Comment Organism
metabolism GDH1 is the protein partner for pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm). Facilitating the recycling of BCATm to form metabolon, GDH1 acts as a catalytic machine Rattus norvegicus