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Literature summary for 1.4.1.3 extracted from

  • Consalvi, V.; Chiaraluce, R.; Politi, L.; Gambacorta, A.; De Rosa, M.; Scandurra, R.
    Glutamate dehydrogenase from the thermoacidophilic archaebacterium Sulfolobus solfataricus. (1991), Eur. J. Biochem., 196, 459-467.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADPH pH 7.6, 60°C Saccharolobus solfataricus
0.14
-
NADH pH 7.6, 50°C Saccharolobus solfataricus
1.4
-
2-oxoglutarate pH 7.6, 60°C Saccharolobus solfataricus
2.5
-
L-glutamate pH 7.6, 60°C Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
6 * 45000, SDS-PAGE Saccharolobus solfataricus
270000
-
gel filtration Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35
-
-
Saccharolobus solfataricus

Storage Stability

Storage Stability Organism
4°C, 1 mg/ml protein concentration, glutamate dehydrogenase remains stable for at least one month Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH specific activity in the presence of NADH is about 30% of that with NADPH Saccharolobus solfataricus L-glutamate + H2O + NAD+
-
r
2-oxoglutarate + NH3 + NADPH
-
Saccharolobus solfataricus L-glutamate + H2O + NADP+
-
r
L-glutamate + H2O + NADP+
-
Saccharolobus solfataricus 2-oxoglutarate + NH3 + NADPH
-
r
norvaline + H2O + NADP+ activity is 20% of that observed in the presence of 2-oxoglutarate and L-glutamate Saccharolobus solfataricus 2-oxovalerate + NH3 + NADPH
-
r

Subunits

Subunits Comment Organism
hexamer 6 * 45000, SDS-PAGE Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
NAD(P)-dependent glutamate dehydrogenase
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
substrates: 2-oxoglutarate + NH3 + NADPH Saccharolobus solfataricus
70
-
substrates: L-glutamate + H2O + NADP+ Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 75 45°C: about 35% of maximal activity, 75°C: about 20% of maximal activity, substrates: 2-oxoglutarate + NH3 + NADPH Saccharolobus solfataricus
60 75 60°C: about 60% of maximal activity, 75°C: about 30% of maximal activity, substrates: L-glutamate + H2O + NADP+ Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
48 h, protein concentration of 0.2 mg/ml, stable Saccharolobus solfataricus
60
-
48 h, protein concentration of 0.2 mg/ml, 20% loss of activity Saccharolobus solfataricus
80
-
half-life: 15 h Saccharolobus solfataricus
85
-
half-life: 2 h Saccharolobus solfataricus
90
-
half-life: 20 min Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
substrates: 2-oxoglutarate + NH3 + NADPH Saccharolobus solfataricus
10
-
substrates: L-glutamate + H2O + NADP+ Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NADH
-
Saccharolobus solfataricus
NADP+
-
Saccharolobus solfataricus
NADPH
-
Saccharolobus solfataricus

pI Value

Organism Comment pI Value Maximum pI Value
Saccharolobus solfataricus isoelectric focusing. Microheterogeneity shows three active bands corresponding to pI of 5.7, 5.5 and 5.4. The major band shows an isoelectric point of 5.7
-
5.7