Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.1.3 extracted from

  • Yang, S.J.; Huh, J.W.; Hong, H.N.; Kim, T.U.; Cho, S.W.
    Important role of Ser443 in different thermal stability of human glutamate dehydrogenase isozymes (2004), FEBS Lett., 562, 59-64.
    View publication on PubMed
Search for more literature for 1.4.1.3

Cloned(Commentary)

Cloned (Comment) Organism
wild-type enzyme hGDH2 and mutant enzymes Homo sapiens
wild-type hGDH1 Homo sapiens

Protein Variants

Protein Variants Comment Organism
A456G mutant of isoenzyme hGDH2 shows no change in heat inactivation process compared to wild-type enzyme Homo sapiens
H470R mutant of isoenzyme hGDH2 shows no change in heat inactivation process compared to wild-type enzyme Homo sapiens
L415M mutant of isoenzyme hGDH2 shows no change in heat inactivation process compared to wild-type enzyme Homo sapiens
S443R mutant of isoenzyme hGDH2 shows a dramatic increase in thermal stability from 45 min at 45°C for the wild-type enzyme to 300 min for the mutant enzyme. KM-values and turnover-numbers are nearly identical to wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.081
-
 NADH pH 8.0, 25°C, wild-type enzyme hGDH1 Homo sapiens
0.086
-
 NADH pH 8.0, 25°C, wild-type enzyme hGDH2 Homo sapiens
0.088
-
 NADH pH 8.0, 25°C, mutant enzyme S443R hGDH2 Homo sapiens
1.25
-
 2-oxoglutarate pH 8.0, 25°C, wild-type enzyme hGDH1 Homo sapiens
1.39
-
 2-oxoglutarate pH 8.0, 25°C, wild-type enzyme hGDH2 Homo sapiens
1.41
-
 2-oxoglutarate pH 8.0, 25°C, mutant enzyme S443R hGDH2 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56500
-
 6 * 56500, hGDH1, SDS-PAGE Homo sapiens
56500
-
 6 * 56500, hGDH2, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NADH + NH3
-
 Homo sapiens L-glutamate + NAD+ + H2O
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 56500, hGDH1, SDS-PAGE Homo sapiens
hexamer 6 * 56500, hGDH2, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
hGDH1
-
 Homo sapiens
hGDH2
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 hGDH1: much slower heat inactivation processes in presence of 1 mM ADP or 3 mM L-Leu Homo sapiens
additional information
-
 much slower heat inactivation processes in presence of 1 mM ADP or 3 mM L-Leu Homo sapiens
45
-
 half-life of hGDH1 is 310 min in absence of allosteric regulators Homo sapiens
45
-
 half-life of hGDH2 is 45 min in absence of allosteric regulators, hGDH2 is stable for 100 min in presence of 3 mM L-Leu and 1 mM ADP, mutant enzyme hGDH2 S443R has a half life of 300 min Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
104
-
 NADH pH 8.0, 25°C, wild-type enzyme hGDH1 Homo sapiens
104
-
 2-oxoglutarate pH 8.0, 25°C, wild-type enzyme hGDH1 Homo sapiens
130
-
 NADH pH 8.0, 25°C, wild-type enzyme hGDH2 Homo sapiens
130
-
 2-oxoglutarate pH 8.0, 25°C, wild-type enzyme hGDH2 Homo sapiens
134
-
 NADH pH 8.0, 25°C, mutant enzyme S443R hGDH2 Homo sapiens
134
-
 2-oxoglutarate pH 8.0, 25°C, mutant enzyme S443R hGDH2 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Homo sapiens