Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | no activation of R463A mutant enzyme | Homo sapiens | |
ATP | wild-type: activation at 1 mM, inhibition below and above, H454Y and S448P mutant enzymes: progressive increase in activity until 10 mM, inhibition above | Homo sapiens | |
leucine | wild-type, S448P, H454Y and R463A mutant enzymes | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
coexpression of wild-type and mutant enzymes with GroES and GroEl in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H454Y | lower basal activity but comparable maximal activity as wild-type | Homo sapiens |
R463A | stimulatory effect of ADP is eliminated | Homo sapiens |
S448P | unstable in Tris-buffer especially in the absence of allosteric activators, basal and maximal specific activity is lower than that from wild-type | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | wild-type: inhibition at 0.1 mM and between 0.5-1.0 mM and above, activation at 1 mM, H454Y and S448P mutant enzyme: activation between 0.1-1 mM, inhibition above, R463A mutant enzyme: progressive inhibition between 0.01 and 10 mM | Homo sapiens | |
diethylstilbestrol | - |
Homo sapiens | |
GTP | little inhibition of H454Y mutant enzyme | Homo sapiens | |
palmitoyl-CoA | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzyme, Q-Sepharose, omega-aminopentyl column, GTP-agarose | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19 | - |
recombinant S448P mutant enzyme, basal activity | Homo sapiens |
55 | - |
recombinant S448P mutant enzyme, maximal activity in the presence of 6 mM leucine | Homo sapiens |
69 | - |
recombinant S448P mutant enzyme, maximal activity in the presence of 0.2 mM ADP | Homo sapiens |
73 | - |
recombinant R463A mutant enzyme, basal activity | Homo sapiens |
74 | - |
recombinant H454Y mutant enzyme, basal activity | Homo sapiens |
84 | - |
recombinant wild-type enzyme, basal activity | Homo sapiens |
100 | - |
recombinant R463A mutant enzyme, maximal activity in the presence of 6 mM leucine | Homo sapiens |
130 | - |
recombinant wild-type enzyme, maximal activity in the presence of 0.2 mM ADP or 6 mM leucine | Homo sapiens |
130 | - |
recombinant S448P mutant enzyme, maximal activity in the presence of 6 mM leucine | Homo sapiens |
140 | - |
recombinant H454Y mutant enzyme, maximal activity in the presence of 0.2 mM ADP | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD(P)+ | - |
Homo sapiens | 2-oxoglutarate + NH3 + NAD(P)H | - |
r |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000013 | - |
palmitoyl-CoA | inhibition of recombinant S448P mutant enzyme | Homo sapiens | |
0.000015 | - |
palmitoyl-CoA | inhibition of recombinant H454Y mutant enzyme | Homo sapiens | |
0.00003 | - |
palmitoyl-CoA | inhibition of recombinant wild-type enzyme | Homo sapiens | |
0.000042 | - |
GTP | inhibition of recombinant wild-type enzyme | Homo sapiens | |
0.0002 | - |
palmitoyl-CoA | inhibition of recombinant R463A mutant enzyme | Homo sapiens | |
0.00022 | - |
GTP | inhibition of recombinant R463A mutant enzyme | Homo sapiens | |
0.00036 | - |
diethylstilbestrol | inhibition of recombinant H454Y mutant enzyme | Homo sapiens | |
0.00074 | - |
diethylstilbestrol | inhibition of recombinant S448P mutant enzyme | Homo sapiens | |
0.0017 | - |
diethylstilbestrol | inhibition of recombinant wild-type enzyme | Homo sapiens | |
0.0031 | - |
GTP | inhibition of recombinant S448P mutant enzyme | Homo sapiens | |
0.0051 | - |
diethylstilbestrol | inhibition of recombinant R463A mutant enzyme | Homo sapiens | |
0.21 | - |
GTP | inhibition of recombinant H454Y mutant enzyme | Homo sapiens |