Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | activates NADH-linked glutamate synthesis, inhibits NADPH-linked activity | Cereibacter sphaeroides | |
AMP | activation only in biosynthetic direction | Cereibacter sphaeroides | |
L-aspartate | 2fold activation of glutamate synthesis | Cereibacter sphaeroides |
General Stability | Organism |
---|---|
AMP stabilizes | Cereibacter sphaeroides |
L-aspartate stabilizes | Cereibacter sphaeroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | inhibits only NADPH-linked activity | Cereibacter sphaeroides | |
ATP | - |
Cereibacter sphaeroides | |
fumarate | - |
Cereibacter sphaeroides | |
L-aspartate | inhibition of NADPH linked reaction, activation of NAD(H) linked reaction | Cereibacter sphaeroides | |
malate | 5 mM, complete inhibition of NADH-linked activity | Cereibacter sphaeroides | |
phosphoenolpyruvate | - |
Cereibacter sphaeroides | |
succinate | 5 mM, complete inhibition of NADH-linked activity | Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
NADH | 50 mM Tris-HCl, pH 7.2 | Cereibacter sphaeroides | |
0.01 | - |
NADH | 500 mM Tris-HCl, pH 8.2 | Cereibacter sphaeroides | |
0.012 | - |
NADH | 500 mM Tris-HCl, pH 7.2 | Cereibacter sphaeroides | |
0.016 | - |
NAD+ | 50 mM Tris-HCl, pH 7.2 | Cereibacter sphaeroides | |
0.11 | - |
NAD+ | 500 mM Tris-HCl, pH 9.4 | Cereibacter sphaeroides | |
0.15 | - |
NAD+ | 500 mM Tris-HCl, pH 7.2 | Cereibacter sphaeroides | |
0.17 | - |
NADPH | 50 mM Tris-HCl, pH 7.2 | Cereibacter sphaeroides | |
0.4 | - |
2-oxoglutarate | 50 mM Tris-HCl, pH 7.2 | Cereibacter sphaeroides | |
0.54 | - |
NADPH | 500 mM Tris-HCl, pH 7.2 | Cereibacter sphaeroides | |
0.75 | - |
NADPH | 500 mM Tris-HCl, pH 8.2 | Cereibacter sphaeroides | |
1.2 | - |
2-oxoglutarate | 500 mM Tris-HCl, pH 7.2 | Cereibacter sphaeroides | |
1.7 | - |
L-glutamate | 50 mM Tris-HCl, pH 7.2 | Cereibacter sphaeroides | |
14 | - |
2-oxoglutarate | 500 mM Tris-HCl, pH 8.2 | Cereibacter sphaeroides | |
19.5 | - |
L-glutamate | 500 mM Tris-HCl, pH 9.4 | Cereibacter sphaeroides | |
31 | - |
L-glutamate | 500 mM Tris-HCl, pH 7.2 | Cereibacter sphaeroides | |
58 | - |
NH4+ | 500 mM Tris-HCl, pH 8.2 | Cereibacter sphaeroides | |
77 | - |
NH4+ | 500 mM Tris-HCl, pH 7.2 | Cereibacter sphaeroides | |
160 | - |
NH4+ | 50 mM Tris-HCl, pH 7.2 | Cereibacter sphaeroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
strain DSM 158 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4 | - |
cofactor NADPH | Cereibacter sphaeroides |
13 | - |
cofactor NADH | Cereibacter sphaeroides |
Storage Stability | Organism |
---|---|
-18°C, several months, no loss of activity | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH3 + NADH + H+ | - |
Cereibacter sphaeroides | L-glutamate + H2O + NAD+ | - |
r | |
L-glutamate + H2O + NAD(P)+ | - |
Cereibacter sphaeroides | 2-oxoglutarate + NH3 + NAD(P)H | - |
r | |
L-glutamate + H2O + NAD+ | - |
Cereibacter sphaeroides | 2-oxoglutarate + NH3 + NADH + H+ | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
NADH + NH3 + 2-oxoglutarate | Cereibacter sphaeroides |
9.3 | - |
NAD+ + glutamate | Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Cereibacter sphaeroides | |
NADH | - |
Cereibacter sphaeroides | |
NADP+ | only 5% of the activity with NAD+ | Cereibacter sphaeroides | |
NADPH | - |
Cereibacter sphaeroides |