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Literature summary for 1.4.1.25 extracted from

  • Li, C.; Lu, C.D.
    Arginine racemization by coupled catabolic and anabolic dehydrogenases (2009), Proc. Natl. Acad. Sci. USA, 106, 906-911.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.25

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HXE4
-
-
Pseudomonas aeruginosa DSM 22644 Q9HXE4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-guanidino-2-oxopentanoate + NH3 + NAD(P)H + H+ DauB uses 2-ketoarginine and ammonia derived from D-arginine in the DauA-catalyzed reaction Pseudomonas aeruginosa L-arginine + H2O + NAD(P)+
-
 ?
5-guanidino-2-oxopentanoate + NH3 + NAD(P)H + H+ DauB uses 2-ketoarginine and ammonia derived from D-arginine in the DauA-catalyzed reaction Pseudomonas aeruginosa DSM 22644 L-arginine + H2O + NAD(P)+
-
 ?
L-arginine + H2O + NAD(P)+
-
 Pseudomonas aeruginosa 5-guanidino-2-oxopentanoate + NH3 + NAD(P)H + H+
-
 r
L-arginine + H2O + NAD(P)+
-
 Pseudomonas aeruginosa DSM 22644 5-guanidino-2-oxopentanoate + NH3 + NAD(P)H + H+
-
 r
additional information no substantial reaction with D-arginine Pseudomonas aeruginosa ?
-
 ?
additional information no substantial reaction with D-arginine Pseudomonas aeruginosa DSM 22644 ?
-
 ?

Synonyms

Synonyms Comment Organism
dauB
-
 Pseudomonas aeruginosa
NAD(P)H-dependent anabolic L-arginine dehydrogenase
-
 Pseudomonas aeruginosa

Expression

Organism Comment Expression
Pseudomonas aeruginosa expression of the DauBAR operon is highly induced in presence of D-arginine up

General Information

General Information Comment Organism
physiological function strains with a lesion at DauA or DauB genes fail to use D-arginine as sole carbon source. Growth complementation of an L-arginine auxotroph by D-arginine is abolished by a lesion at DauA or DauB. Presence of D-arginine induces L-arginine-specific genes in the parental strain PAO1 but not in its dauA or dauB mutants. DauA catalyzes oxidative deamination of D-arginine into 2-ketoarginine and ammonia, and DauB is able to use 2-ketoarginine and ammonia as substrates and convert them into L-arginine in the presence of NADPH or NADH Pseudomonas aeruginosa