Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 0.1 mM, strong inhibition | Streptomyces virginiae | |
Ca2+ | 1 mM, partial inhibition | Streptomyces virginiae | |
Cu2+ | 0.1 mM, strong inhibition | Streptomyces virginiae | |
Hg+ | 0.1 mM, strong inhibition | Streptomyces virginiae | |
Hg2+ | 0.1 mM, strong inhibition | Streptomyces virginiae | |
Mg2+ | 1 mM, partial inhibition | Streptomyces virginiae | |
additional information | not inhibitory: EDTA, benzamidine, PMSF, all at 5 mM, or citrate, cobalt(II), azide, Li+ and Mn2+, at 1 mM | Streptomyces virginiae | |
p-chloromercuribenzoate | 0.1 mM, strong inhibition | Streptomyces virginiae | |
Zn2+ | 1 mM, partial inhibition | Streptomyces virginiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
2-oxobutanoate | pH 9.5, 21°C | Streptomyces virginiae | |
0.48 | - |
2-oxovalerate | pH 9.5, 21°C | Streptomyces virginiae | |
0.5 | - |
2-Oxoisohexanoate | pH 9.5, 21°C | Streptomyces virginiae | |
0.81 | - |
2-oxoisovalerate | pH 9.5, 21°C | Streptomyces virginiae | |
0.81 | - |
2-Oxohexanoate | pH 9.5, 21°C | Streptomyces virginiae | |
1.3 | - |
L-valine | pH 10.0, 21°C | Streptomyces virginiae | |
3.2 | - |
L-norvaline | pH 10.0, 21°C | Streptomyces virginiae | |
4 | - |
L-leucine | pH 10.0, 21°C | Streptomyces virginiae | |
10.8 | - |
L-isoleucine | pH 10.0, 21°C | Streptomyces virginiae | |
12.3 | - |
pyruvate | pH 9.5, 21°C | Streptomyces virginiae | |
19.6 | - |
L-2-aminobutanoate | pH 10.0, 21°C | Streptomyces virginiae | |
30 | - |
L-norleucine | pH 10.0, 21°C | Streptomyces virginiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
2 * 41000, SDS-PAGE | Streptomyces virginiae |
88000 | - |
gel filtration | Streptomyces virginiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces virginiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces virginiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-valine + H2O + NAD+ = 3-methyl-2-oxobutanoate + NH3 + NADH + H+ | the reductive amination proceeds through a sequential ordered ternary-binary mechanism. NADH binds to the enzyme first, followed by 2-oxoisovalerate and NH3, and valine is released first, followed by NAD+ | Streptomyces virginiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutanoate + NH3 + NADH + H+ | - |
Streptomyces virginiae | L-2-aminobutanoate + H2O + NAD+ | - |
r | |
2-oxohexanoate + NH3 + NADH + H+ | - |
Streptomyces virginiae | L-norleucine + H2O + NAD+ | - |
r | |
2-oxoisohexanoate + NH3 + NADH + H+ | - |
Streptomyces virginiae | L-leucine + H2O + NAD+ | - |
r | |
2-oxoisovalerate + NH3 + NADH + H+ | - |
Streptomyces virginiae | L-valine + H2O + NAD+ | the 4'-pro-S hydrogen atom of NADH is transferred to the substrate in the reductive-amination reaction | r | |
2-oxovalerate + H2O + NAD+ | - |
Streptomyces virginiae | L-norvaline + NH3 + NADH + H+ | - |
r | |
3-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
Streptomyces virginiae | L-isoleucine + H2O + NAD+ | - |
r | |
L-2-aminobutanoate + H2O + NAD+ | - |
Streptomyces virginiae | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-alanine + H2O + NAD+ | - |
Streptomyces virginiae | pyruvate + NH3 + NADH + H+ | - |
r | |
L-isoleucine + H2O + NAD+ | - |
Streptomyces virginiae | 3-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
L-leucine + H2O + NAD+ | - |
Streptomyces virginiae | 2-oxoisohexanoate + NH3 + NADH + H+ | - |
r | |
L-norleucine + H2O + NAD+ | - |
Streptomyces virginiae | 2-oxohexanoate + NH3 + NADH + H+ | - |
r | |
L-norvaline + H2O + NAD+ | - |
Streptomyces virginiae | 2-oxovalerate + NH3 + NADH + H+ | - |
r | |
L-valine + H2O + NAD+ | - |
Streptomyces virginiae | 3-methyl-2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
pyruvate + NH3 + NADH + H+ | - |
Streptomyces virginiae | L-alanine + H2O + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41000, SDS-PAGE | Streptomyces virginiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.8 | - |
L-isoleucine | pH 10.0, 21°C | Streptomyces virginiae | |
11.9 | - |
L-leucine | pH 10.0, 21°C | Streptomyces virginiae | |
17.9 | - |
L-norleucine | pH 10.0, 21°C | Streptomyces virginiae | |
18 | - |
L-norvaline | pH 10.0, 21°C | Streptomyces virginiae | |
28.3 | - |
L-valine | pH 10.0, 21°C | Streptomyces virginiae | |
71.9 | - |
2-Oxoisohexanoate | pH 9.5, 21°C | Streptomyces virginiae | |
99 | - |
2-oxobutanoate | pH 9.5, 21°C | Streptomyces virginiae | |
104 | - |
L-2-aminobutanoate | pH 10.0, 21°C | Streptomyces virginiae | |
107 | - |
2-Oxohexanoate | pH 9.5, 21°C | Streptomyces virginiae | |
114 | - |
pyruvate | pH 9.5, 21°C | Streptomyces virginiae | |
164 | - |
2-oxovalerate | pH 9.5, 21°C | Streptomyces virginiae | |
226 | - |
2-oxoisovalerate | pH 9.5, 21°C | Streptomyces virginiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
reductive amination | Streptomyces virginiae |
10 | - |
oxidative deamination | Streptomyces virginiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
dramatic decrease in stability below | Streptomyces virginiae |
7.4 | - |
most stable at | Streptomyces virginiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor: NADP+ | Streptomyces virginiae | |
NAD+ | - |
Streptomyces virginiae | |
NADH | - |
Streptomyces virginiae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptomyces virginiae | isoelectric focusing | - |
6.2 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
L-isoleucine | pH 10.0, 21°C | Streptomyces virginiae | |
0.6 | - |
L-norleucine | pH 10.0, 21°C | Streptomyces virginiae | |
3 | - |
L-leucine | pH 10.0, 21°C | Streptomyces virginiae | |
5.3 | - |
L-2-aminobutanoate | pH 10.0, 21°C | Streptomyces virginiae | |
5.6 | - |
L-norvaline | pH 10.0, 21°C | Streptomyces virginiae | |
9.3 | - |
pyruvate | pH 9.5, 21°C | Streptomyces virginiae | |
21.8 | - |
L-valine | pH 10.0, 21°C | Streptomyces virginiae | |
131 | - |
2-Oxohexanoate | pH 9.5, 21°C | Streptomyces virginiae | |
143 | - |
2-Oxoisohexanoate | pH 9.5, 21°C | Streptomyces virginiae | |
279 | - |
2-oxoisovalerate | pH 9.5, 21°C | Streptomyces virginiae | |
340 | - |
2-oxovalerate | pH 9.5, 21°C | Streptomyces virginiae | |
740 | - |
2-oxobutanoate | pH 9.5, 21°C | Streptomyces virginiae |