General Stability | Organism |
---|---|
enzyme activity in Tris-HCl buffer is about 7fold higher than in potassium phosphate buffer | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Klebsiella pneumoniae | |
K+ | potassium ion in phosphate buffer may be inhibitory | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
2 * 62000, SDS-PAGE | Klebsiella pneumoniae |
124000 | - |
gel filtration | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + NADP+ + H2O | Klebsiella pneumoniae | - |
oxaloacetate + NH4+ + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
vitamin B12-producing strain IFO 13541 | - |
Purification (Comment) | Organism |
---|---|
about 500fold | Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.045 | - |
pH 7, 30°C, crude extract | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + NADP+ + H2O | - |
Klebsiella pneumoniae | oxaloacetate + NH4+ + NADPH | - |
? | |
additional information | not: D-aspartate, L-glutamate, L-glycine, L-alanine, L-threonine, L-serine, L-leucine, L-isoleucine, L-methionine, L-cysteine, L-proline, L-valine, L-phenylalanine, L-tyrosine, L-tryptophan, L-lysine, L-histidine, L-arginine | Klebsiella pneumoniae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 62000, SDS-PAGE | Klebsiella pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
NAD-dependent aspartate dehydrogenase | - |
Klebsiella pneumoniae |
NADH2-dependent aspartate dehydrogenase | - |
Klebsiella pneumoniae |
NADP+-dependent aspartate dehydrogenase | - |
Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Klebsiella pneumoniae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
up to 20°C, 10 min, Tris-HCl buffer, pH 7.5, stable | Klebsiella pneumoniae |
50 | - |
10 min, Tris-HCl buffer, pH 7.5, 70% loss of activity | Klebsiella pneumoniae |
60 | - |
10 min, Tris-HCl buffer, pH 7.5, 100% loss of activity | Klebsiella pneumoniae |
100 | - |
boiling inactivates | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
in Tris-HCl buffer, oxidative deamination of L-aspartate | Klebsiella pneumoniae |
8 | - |
in potassium phosphate buffer, oxidative deamination of L-aspartate | Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | not: NAD+, FMN, FAD | Klebsiella pneumoniae | |
NADP+ | requires NADP+ as coenzyme | Klebsiella pneumoniae |