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Literature summary for 1.4.1.20 extracted from

  • Shahbaz Mohammadi, H.; Omidinia, E.; Taherkhani, H.
    Rapid one-step separation and purification of recombinant phenylalanine dehydrogenase in aqueous two-phase systems (2008), Iran. Biomed. J., 12, 115-122.
    View publication on PubMed
Search for more literature for 1.4.1.20

Application

Application Comment Organism
medicine plays an important role in detection and screening of phenylketonuria diseases Lysinibacillus sphaericus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Lysinibacillus sphaericus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 NAD+
-
 Lysinibacillus sphaericus
0.21
-
 L-Phe
-
 Lysinibacillus sphaericus

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
polyethylene glycol and ammonium sulfate aequous two-phase purification system with 9% (w/w) PEG-6000 and 16% (w/w) ammonium sulfate at pH 8.0 Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the 491fold purified enzyme exhibits a specific activity of 9828.88 units/mg Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Phe + H2O + NAD+
-
 Lysinibacillus sphaericus phenylpyruvate + NH3 + NADH
-
 ?

Synonyms

Synonyms Comment Organism
PheDH
-
 Lysinibacillus sphaericus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Lysinibacillus sphaericus