Application | Comment | Organism |
---|---|---|
medicine | plays an important role in detection and screening of phenylketonuria diseases | Lysinibacillus sphaericus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Lysinibacillus sphaericus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
NAD+ | - |
Lysinibacillus sphaericus | |
0.21 | - |
L-Phe | - |
Lysinibacillus sphaericus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
polyethylene glycol and ammonium sulfate aequous two-phase purification system with 9% (w/w) PEG-6000 and 16% (w/w) ammonium sulfate at pH 8.0 | Lysinibacillus sphaericus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the 491fold purified enzyme exhibits a specific activity of 9828.88 units/mg | Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Phe + H2O + NAD+ | - |
Lysinibacillus sphaericus | phenylpyruvate + NH3 + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PheDH | - |
Lysinibacillus sphaericus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Lysinibacillus sphaericus |