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Literature summary for 1.4.1.20 extracted from
- Glycosidation of phenylalanine dehydrogenase with O-carboxymethyl-poly-beta-cyclodextrin (2007), Enzyme Microb. Technol., 40, 471-475.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21/pBBNH cells | Bacillus badius |
General Stability
| General Stability | Organism |
|---|---|
| glycosylation increases temperature stability | Bacillus badius |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.186 | - |
L-Phe | PheDH after glycosidation | Bacillus badius |  |
| 0.215 | - |
L-Phe | unmodified PheDH | Bacillus badius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 326000 | - |
SDS-PAGE, unmodified PheDH | Bacillus badius |
| 720000 | - |
SDS-PAGE, neoglyoenzyme | Bacillus badius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus badius | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the neoglycoenzyme retains 63% of its initial activity and contains about 2.5 mol of O-carboxymethyl poly-beta-cyclodextrin per mole of enzyme | Bacillus badius |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-affinity and anion exchange column chromatography | Bacillus badius |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 450 | - |
PheDH after glycosidation | Bacillus badius |
| 816 | - |
unmodified PheDH | Bacillus badius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Phe + H2O + NAD+ | - |
Bacillus badius | phenylpyruvate + NH3 + NADH | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PheDH | - |
Bacillus badius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
unmodified PheDH | Bacillus badius |
| 55 | - |
neoglycoenzyme | Bacillus badius |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | - |
Bacillus badius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 61 | the optimum temperature for the glycosylated enzyme is increased by 15°C and its thermostability is improved by about 6°C over 10 min incubation, the conjugate is more resistant to thermal inactivation at different temperatures, ranging from 45 to 60°C, the T50 value is increased from 54 to 61°C for PheDH after glycosidation | Bacillus badius |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Bacillus badius | |
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Release 2023.1 (January 2023)
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