Protein Variants | Comment | Organism |
---|---|---|
L307V | shows enhanced pH stability compared to the wild type enzyme | Lysinibacillus sphaericus |
N145A | shows enhanced pH stability compared to the wild type enzyme | Lysinibacillus sphaericus |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | Vmax values decrease substantially in the presence of 10% ethanol | Lysinibacillus sphaericus |
Methanol | Vmax values decrease substantially in the presence of 10% (v/v) methanol | Lysinibacillus sphaericus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-chloro-L-phenylalanine + H2O + NAD+ | - |
Lysinibacillus sphaericus | 4-chloro-phenylpyruvate + NH3 + NADH | - |
? | |
4-fluoro-L-phenylalanine + H2O + NAD+ | - |
Lysinibacillus sphaericus | 4-fluoro-phenylpyruvate + NH3 + NADH | - |
? | |
4-methoxy-L-phenylalanine + H2O + NAD+ | - |
Lysinibacillus sphaericus | 4-methoxy-phenylpyruvate + NH3 + NADH | - |
? | |
4-methyl-L-phenylalanine + H2O + NAD+ | - |
Lysinibacillus sphaericus | 4-methyl-phenylpyruvate + NH3 + NADH | - |
? | |
4-nitro-L-phenylalanine + H2O + NAD+ | - |
Lysinibacillus sphaericus | 4-nitro-phenylpyruvate + NH3 + NADH | - |
? | |
L-Phe + H2O + NAD+ | - |
Lysinibacillus sphaericus | phenylpyruvate + NH3 + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PheDH | - |
Lysinibacillus sphaericus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 10.4 | in pH 8.0 buffer with either 10% methanol or 10% ethanol L307V PheDH retains almost 85% original activity after 7 days, therafter activity continues to decrease gradually, most rapidly in pH 10.4 buffer with 10% ethanol, wild type PheDH is similarly stable, mutant N145A PheDH shows 68% retention of activity after 7 days at pH 8.0 with 10% methanol | Lysinibacillus sphaericus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Lysinibacillus sphaericus |