Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.1.20 extracted from

  • Chen, S.; Engel, P.C.
    An engineered mutant, L307V of phenylalanine dehydrogenase from Bacillus sphaericus: high activity and stability in organic-aqueous solvent mixtures and utility for synthesis of non-natural L-amino acids (2007), Enzyme Microb. Technol., 40, 1407-1411.
No PubMed abstract available
Search for more literature for 1.4.1.20

Protein Variants

Protein Variants Comment Organism
L307V shows enhanced pH stability compared to the wild type enzyme Lysinibacillus sphaericus
N145A shows enhanced pH stability compared to the wild type enzyme Lysinibacillus sphaericus

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol Vmax values decrease substantially in the presence of 10% ethanol Lysinibacillus sphaericus
Methanol Vmax values decrease substantially in the presence of 10% (v/v) methanol Lysinibacillus sphaericus

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-chloro-L-phenylalanine + H2O + NAD+
-
 Lysinibacillus sphaericus 4-chloro-phenylpyruvate + NH3 + NADH
-
 ?
4-fluoro-L-phenylalanine + H2O + NAD+
-
 Lysinibacillus sphaericus 4-fluoro-phenylpyruvate + NH3 + NADH
-
 ?
4-methoxy-L-phenylalanine + H2O + NAD+
-
 Lysinibacillus sphaericus 4-methoxy-phenylpyruvate + NH3 + NADH
-
 ?
4-methyl-L-phenylalanine + H2O + NAD+
-
 Lysinibacillus sphaericus 4-methyl-phenylpyruvate + NH3 + NADH
-
 ?
4-nitro-L-phenylalanine + H2O + NAD+
-
 Lysinibacillus sphaericus 4-nitro-phenylpyruvate + NH3 + NADH
-
 ?
L-Phe + H2O + NAD+
-
 Lysinibacillus sphaericus phenylpyruvate + NH3 + NADH
-
 ?

Synonyms

Synonyms Comment Organism
PheDH
-
 Lysinibacillus sphaericus

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 10.4 in pH 8.0 buffer with either 10% methanol or 10% ethanol L307V PheDH retains almost 85% original activity after 7 days, therafter activity continues to decrease gradually, most rapidly in pH 10.4 buffer with 10% ethanol, wild type PheDH is similarly stable, mutant N145A PheDH shows 68% retention of activity after 7 days at pH 8.0 with 10% methanol Lysinibacillus sphaericus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Lysinibacillus sphaericus