Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-Phe | - |
Rhodococcus sp. | |
L-Phe | - |
Rhodococcus sp. | |
L-Phenyllactate | - |
Rhodococcus sp. | |
NADH | - |
Rhodococcus sp. | |
NH4+ | - |
Rhodococcus sp. | |
Phenylethylamine | - |
Rhodococcus sp. | |
phenylpropionate | - |
Rhodococcus sp. | |
phenylpyruvate | - |
Rhodococcus sp. | |
trans-cinnamate | - |
Rhodococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
40 | - |
oxidized beta-nicotinamide hypoxanthine dinucleotide | - |
Rhodococcus sp. | |
50 | - |
3-pyridinealdehyde-NAD+ | - |
Rhodococcus sp. | |
60 | - |
thioNAD+ | - |
Rhodococcus sp. | |
90 | - |
NAD+ | - |
Rhodococcus sp. | |
110 | - |
3-acetylpyridine-NAD+ | - |
Rhodococcus sp. | |
170 | - |
oxidized beta-nicotinamide guanine dinucleotide | - |
Rhodococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39500 | - |
4 * 39500, SDS-PAGE | Rhodococcus sp. |
150000 | - |
gel filtration | Rhodococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-phenylalanine + H2O + NAD+ = phenylpyruvate + NH3 + NADH + H+ | kinetic mechanism is ordered with NAD+ binding prior to Phe and the products being released in the order of ammonia, phenylpyruvate, and NADH | Rhodococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Phe + H2O + NAD+ | - |
Rhodococcus sp. | phenylpyruvate + NH3 + NADH | - |
r | |
L-phenylalanine + H2O + 3-acetylpyridine-NAD+ | 350% of the activity with NAD+ | Rhodococcus sp. | phenylpyruvate + NH3 + acetylpyridine-NADH | - |
? | |
L-phenylalanine + H2O + 3-pyridinealdehyde-NAD+ | 55% of the activity with NAD+ | Rhodococcus sp. | phenylpyruvate + NH3 + 3-pyridinealdehyde-NADH | - |
? | |
L-phenylalanine + H2O + oxidized beta-nicotinamide guanine dinucleotide | at 86% of the activity with NAD+ | Rhodococcus sp. | phenylpyruvate + NH3 + reduced beta-nicotinamide guanine dinucleotide | - |
? | |
L-phenylalanine + H2O + oxidized beta-nicotinamide hypoxanthine dinucleotide | at 86% of the activity with NAD+ | Rhodococcus sp. | phenylpyruvate + NH3 + reduced beta-nicotinamide hypoxanthine dinucleotide | - |
? | |
L-phenylalanine + H2O + thio-NAD+ | at 86% of the activity with NAD+ | Rhodococcus sp. | phenylpyruvate + NH3 + thio-NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 39500, SDS-PAGE | Rhodococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADH | Rhodococcus sp. | |
NAD+ | - |
Rhodococcus sp. | |
NADH | - |
Rhodococcus sp. |