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Literature summary for 1.4.1.20 extracted from

  • Asano, Y.; Tanetani, M.
    Thermostable phenylalanine dehydrogenase from a mesophilic Microbacterium sp. strain DM 86-1 (1998), Arch. Microbiol., 169, 220-224.
    View publication on PubMed
Search for more literature for 1.4.1.20

Inhibitors

Inhibitors Comment Organism Structure
HgCl2 1.0 mM, 97% inhibition Microbacterium sp.
PCMB 1.0 mM, 98% loss of activity Microbacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 NADH
-
 Microbacterium sp.
0.1
-
 L-Phe
-
 Microbacterium sp.
0.2
-
 NAD+
-
 Microbacterium sp.
0.3
-
 phenylpyruvate
-
 Microbacterium sp.
1.54
-
 L-norleucine
-
 Microbacterium sp.
1.6
-
 L-Met
-
 Microbacterium sp.
85
-
 NH3
-
 Microbacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 8 * 41000, SDS-PAGE Microbacterium sp.
330000
-
 gel filtration Microbacterium sp.

Organism

Organism UniProt Comment Textmining
Microbacterium sp.
-
-
-
Microbacterium sp. DM 86-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Microbacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
37.1
-
-
 Microbacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Leu + H2O + NAD+
-
 Microbacterium sp. 3-methyl-2-oxopentanoic acid + NH3 + NADH
-
 ?
L-Met + H2O + NAD+
-
 Microbacterium sp. 2-oxo-4-methylthiobutanoate + NH3 + NADH
-
 ?
L-norleucine + H2O + NAD+
-
 Microbacterium sp. 2-oxohexanoic acid + NADH + NH3
-
 ?
L-norleucine + H2O + NAD+
-
 Microbacterium sp. DM 86-1 2-oxohexanoic acid + NADH + NH3
-
 ?
L-norvaline + H2O + NAD+
-
 Microbacterium sp. 2-oxopentanoate + NH3 + NADH
-
 ?
L-norvaline + H2O + NAD+
-
 Microbacterium sp. DM 86-1 2-oxopentanoate + NH3 + NADH
-
 ?
L-Phe + H2O + NAD+
-
 Microbacterium sp. phenylpyruvate + NH3 + NADH
-
 r
L-Phe + H2O + NAD+
-
 Microbacterium sp. DM 86-1 phenylpyruvate + NH3 + NADH
-
 r
L-Tyr + H2O + NAD+
-
 Microbacterium sp. 4-hydroxyphenylpyruvate + NH3 + NADH
-
 ?
L-Tyr + H2O + NAD+
-
 Microbacterium sp. DM 86-1 4-hydroxyphenylpyruvate + NH3 + NADH
-
 ?
L-Val + H2O + NAD+
-
 Microbacterium sp. 3-methyl-2-oxobutanoate + NH3 + NADH
-
 ?
L-Val + H2O + NAD+
-
 Microbacterium sp. DM 86-1 3-methyl-2-oxobutanoate + NH3 + NADH
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 41000, SDS-PAGE Microbacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Microbacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 10 min, stable Microbacterium sp.
60
-
 pH 7.0, 10 min, stable Microbacterium sp.
65 70 pH 7.0, 10 min, 50% loss of activity Microbacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
12
-
 oxidative deamination and reductive amination Microbacterium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 12.5 30°C, 30 min, stable Microbacterium sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Microbacterium sp.
NADH
-
 Microbacterium sp.