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Literature summary for 1.4.1.20 extracted from

  • De Boer, L.; Van Rijssel, M.; Euverink, G.J.; Dijkhuizen, L.
    Purification, characterization and regulation of a monomeric L-phenylalanine dehydrogenase from the facultative methylotroph Nocardia sp. 239 (1989), Arch. Microbiol., 153, 12-18.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
phenylpyruvate
-
Nocardia sp.
0.23
-
NAD+
-
Nocardia sp.
0.75
-
L-Phe
-
Nocardia sp.
96
-
NH3
-
Nocardia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
gel filtration Nocardia sp.

Organism

Organism UniProt Comment Textmining
Nocardia sp.
-
239
-
Nocardia sp. 239
-
239
-

Purification (Commentary)

Purification (Comment) Organism
partial Nocardia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxohexanoate + NH3 + NADH 240% of the activity with phenylpyruvate Nocardia sp. 2-aminohexanoate + H2O + NAD+
-
?
2-oxohexanoate + NH3 + NADH 240% of the activity with phenylpyruvate Nocardia sp. 239 2-aminohexanoate + H2O + NAD+
-
?
4-hydroxyphenylpyruvate + NH3 + NADH 28% of the activity with phenylpyruvate Nocardia sp. L-Tyr + NAD+ + H2O
-
?
4-hydroxyphenylpyruvate + NH3 + NADH 28% of the activity with phenylpyruvate Nocardia sp. 239 L-Tyr + NAD+ + H2O
-
?
indole-3-pyruvate + NH3 + NADH + H+ 54% of the activity with phenylpyruvate Nocardia sp. L-Trp + NAD+ + H2O
-
?
indole-3-pyruvate + NH3 + NADH + H+ 54% of the activity with phenylpyruvate Nocardia sp. 239 L-Trp + NAD+ + H2O
-
?
L-Leu + H2O + NAD+
-
Nocardia sp. 3-methyl-2-oxopentanoic acid + NH3 + NADH
-
?
L-Leu + H2O + NAD+
-
Nocardia sp. 239 3-methyl-2-oxopentanoic acid + NH3 + NADH
-
?
L-Phe + H2O + NAD+
-
Nocardia sp. phenylpyruvate + NH3 + NADH
-
r
L-Phe + H2O + NAD+
-
Nocardia sp. 239 phenylpyruvate + NH3 + NADH
-
r

Subunits

Subunits Comment Organism
monomer 1 * 42000, SDS-PAGE Nocardia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
53
-
-
Nocardia sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 53 40°C: about 65% of maximal activity, 53°C: optimum, no activity at 55°C Nocardia sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
53
-
pH 6.0, 5 min, 50% loss of activity Nocardia sp.
53
-
phenylpyruvate destabilizes the activity, 1 M sorbitol or 1 M glycerol stabilize Nocardia sp.
53
-
pH 9.5-10, 2 h, 50% loss of activity Nocardia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Nocardia sp.

pH Range

pH Minimum pH Maximum Comment Organism
9.3 10.2 pH 9.3: about 40% of maximal activity, pH 10.2: about 35% of maximal activity Nocardia sp.

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADP+ Nocardia sp.
NAD+
-
Nocardia sp.
NADH
-
Nocardia sp.