KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
phenylpyruvate | - |
Nocardia sp. | |
0.23 | - |
NAD+ | - |
Nocardia sp. | |
0.75 | - |
L-Phe | - |
Nocardia sp. | |
96 | - |
NH3 | - |
Nocardia sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
gel filtration | Nocardia sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardia sp. | - |
239 | - |
Nocardia sp. 239 | - |
239 | - |
Purification (Comment) | Organism |
---|---|
partial | Nocardia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxohexanoate + NH3 + NADH | 240% of the activity with phenylpyruvate | Nocardia sp. | 2-aminohexanoate + H2O + NAD+ | - |
? | |
2-oxohexanoate + NH3 + NADH | 240% of the activity with phenylpyruvate | Nocardia sp. 239 | 2-aminohexanoate + H2O + NAD+ | - |
? | |
4-hydroxyphenylpyruvate + NH3 + NADH | 28% of the activity with phenylpyruvate | Nocardia sp. | L-Tyr + NAD+ + H2O | - |
? | |
4-hydroxyphenylpyruvate + NH3 + NADH | 28% of the activity with phenylpyruvate | Nocardia sp. 239 | L-Tyr + NAD+ + H2O | - |
? | |
indole-3-pyruvate + NH3 + NADH + H+ | 54% of the activity with phenylpyruvate | Nocardia sp. | L-Trp + NAD+ + H2O | - |
? | |
indole-3-pyruvate + NH3 + NADH + H+ | 54% of the activity with phenylpyruvate | Nocardia sp. 239 | L-Trp + NAD+ + H2O | - |
? | |
L-Leu + H2O + NAD+ | - |
Nocardia sp. | 3-methyl-2-oxopentanoic acid + NH3 + NADH | - |
? | |
L-Leu + H2O + NAD+ | - |
Nocardia sp. 239 | 3-methyl-2-oxopentanoic acid + NH3 + NADH | - |
? | |
L-Phe + H2O + NAD+ | - |
Nocardia sp. | phenylpyruvate + NH3 + NADH | - |
r | |
L-Phe + H2O + NAD+ | - |
Nocardia sp. 239 | phenylpyruvate + NH3 + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42000, SDS-PAGE | Nocardia sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
53 | - |
- |
Nocardia sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 53 | 40°C: about 65% of maximal activity, 53°C: optimum, no activity at 55°C | Nocardia sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
53 | - |
pH 6.0, 5 min, 50% loss of activity | Nocardia sp. |
53 | - |
phenylpyruvate destabilizes the activity, 1 M sorbitol or 1 M glycerol stabilize | Nocardia sp. |
53 | - |
pH 9.5-10, 2 h, 50% loss of activity | Nocardia sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Nocardia sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9.3 | 10.2 | pH 9.3: about 40% of maximal activity, pH 10.2: about 35% of maximal activity | Nocardia sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADP+ | Nocardia sp. | |
NAD+ | - |
Nocardia sp. | |
NADH | - |
Nocardia sp. |