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Literature summary for 1.4.1.20 extracted from

  • Asano, Y.; Nakazawa, A.; Endo, K.
    Novel phenylalanine dehydrogenases from Sporosarcina ureae and Bacillus sphaericus. Purification and characterization (1987), J. Biol. Chem., 262, 10346-10354.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Lysinibacillus sphaericus
-
Sporosarcina ureae

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithio-bis(2-nitrobenzoic acid) 0.17 mM: 26% inhibition Lysinibacillus sphaericus
5,5'-dithio-bis(2-nitrobenzoic acid) 0.17 mM, complete inhibition Sporosarcina ureae
AgNO3 1 mM, complete inhibition Lysinibacillus sphaericus
AgNO3 1 mM, complete inhibition Sporosarcina ureae
D-Leu 10 mM, 17% inhibition Lysinibacillus sphaericus
D-Leu 10 mM, 31% inhibition Sporosarcina ureae
D-Met 10 mM, 63% inhibition Lysinibacillus sphaericus
D-Met 10 mM, 25% inhibition Sporosarcina ureae
D-norleucine 10 mM, 52% inhibition Lysinibacillus sphaericus
D-norleucine 10 mM, 34% inhibition Sporosarcina ureae
D-Phe 10 mM, 89% inhibition Lysinibacillus sphaericus
D-Phe 10 mM, 77% inhibition Sporosarcina ureae
D-Trp 5 mM, 83% inhibition Lysinibacillus sphaericus
D-Trp 5 mM, 65% inhibition Sporosarcina ureae
D-Tyr 1 mM, 29% inhibition Lysinibacillus sphaericus
D-Val 10 mM, 11% inhibition Lysinibacillus sphaericus
HgCl2 0.01 mM, 62% inhibition Lysinibacillus sphaericus
HgCl2 0.01 M, complete inhibition Sporosarcina ureae
L-Cys 10 mM, 41% inhibition Lysinibacillus sphaericus
L-Ile 10 mM, 75% inhibition Lysinibacillus sphaericus
L-Leu 10 mM, 58% inhibition Lysinibacillus sphaericus
L-Leu 10 mM, 39% inhibition Sporosarcina ureae
L-Val 10 mM, 23% inhibition Lysinibacillus sphaericus
NEM 1 mM: 79% inhibition Sporosarcina ureae
PCMB 0.02 mM, complete inhibition Lysinibacillus sphaericus
PCMB 0.02 mM, complete inhibition Sporosarcina ureae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
NADH
-
Lysinibacillus sphaericus
0.072
-
NADH
-
Sporosarcina ureae
0.14
-
NAD+
-
Sporosarcina ureae
0.16
-
phenylpyruvate
-
Sporosarcina ureae
0.17
-
NAD+
-
Sporosarcina ureae
0.34
-
4-hydroxyphenylpyruvate
-
Sporosarcina ureae
0.4
-
phenylpyruvate
-
Sporosarcina ureae
2.44
-
2-Oxohexanoate
-
Sporosarcina ureae
78
-
NH3
-
Lysinibacillus sphaericus
85
-
NH3
-
Sporosarcina ureae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
305000
-
equilibrium sedimentation Sporosarcina ureae
310000
-
gel filtration Sporosarcina ureae
340000
-
gel filtration, equilibrium sedimentation Lysinibacillus sphaericus

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus
-
-
-
Sporosarcina ureae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lysinibacillus sphaericus
-
Sporosarcina ureae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
87.9
-
-
Sporosarcina ureae
111
-
-
Lysinibacillus sphaericus

Storage Stability

Storage Stability Organism
4°C, 0.01 M potassium phosphate buffer, pH 7.0, 0.1 mM EDTA, 5 mM 2-mercaptoethanol, stable for at least 2 years in crystalline form Lysinibacillus sphaericus
4°C, 0.01 M potassium phosphate buffer, pH 7.0, 0.1 mM EDTA, 5 mM 2-mercaptoethanol, stable for at least 2 years in crystalline form Sporosarcina ureae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxohexanoate + NH3 + NADH
-
Sporosarcina ureae 2-aminohexanoate + H2O + NAD+
-
?
4-hydroxyphenylpyruvate + NH3 + NADH
-
Lysinibacillus sphaericus L-Tyr + NAD+ + H2O
-
?
4-hydroxyphenylpyruvate + NH3 + NADH + H+
-
Sporosarcina ureae L-tyrosine + NAD+ + H2O
-
r
L-norleucine + H2O + NAD+
-
Sporosarcina ureae 2-oxohexanoic acid + NADH + NH3
-
?
L-Phe + H2O + NAD+
-
Lysinibacillus sphaericus phenylpyruvate + NH3 + NADH
-
r
L-Phe + H2O + NAD+
-
Sporosarcina ureae phenylpyruvate + NH3 + NADH
-
r
L-phenylalanine methyl ester + H2O + NAD+
-
Sporosarcina ureae phenylpyruvic acid methyl ester + NH3 + NADH
-
?
L-Tyr + H2O + NAD+
-
Lysinibacillus sphaericus 4-hydroxyphenylpyruvate + NH3 + NADH
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Sporosarcina ureae
50
-
-
Lysinibacillus sphaericus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
pH 9.0, stable Sporosarcina ureae
40
-
pH 9.0, 10 min, 25% loss of activity Sporosarcina ureae
50
-
pH 9.0, 10 min, complete inactivation Sporosarcina ureae
55
-
pH 9.0, 10 min, stable Lysinibacillus sphaericus
60
-
pH 9.0, 10 min, most of the activity is lost Lysinibacillus sphaericus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
reductive amination of phenylpyruvate Sporosarcina ureae
10.3
-
reductive amination of phenylpyruvate Sporosarcina ureae
10.5
-
oxidative deamination of L-Phe Sporosarcina ureae
11.3
-
oxidative deamination of L-Phe Sporosarcina ureae

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
30°C, 1 h, less than 20% loss of activity Lysinibacillus sphaericus
9
-
30°C, 1 h, stable Lysinibacillus sphaericus
9
-
30°C, 1 h, stable Sporosarcina ureae
11.3
-
30°C, 1 h, stable Lysinibacillus sphaericus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Lysinibacillus sphaericus
NAD+
-
Sporosarcina ureae
NADH
-
Lysinibacillus sphaericus
NADH
-
Sporosarcina ureae