Literature summary for 1.4.1.2 extracted from

Oliveira, T.F.; Carrigan, J.B.; Hamza, M.A.; Sharkey, M.A.; Engel, P.C.; Khan, A.R.
Crystallization and preliminary structural analyses of glutamate dehydrogenase from Peptoniphilus asaccharolyticus (2010), Acta Crystallogr. Sect. F, 66, 523-526.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type GDH in Escherichia coli strain BL21(DE3), and of the SeMet-labeled enzyme in Escherichia coli strain B834 (DE3)	Peptoniphilus asaccharolyticus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant wild-type and SeMet-labeled GDHs, hanging drop vapour diffusion method, at 20°C, protein in 10 mM Tris-HCl, pH 7.0, mixing of 0.002 ml of protein solution with 0.001 ml of reservoir solution containing 2.0 M ammonium sulfate, 0.1 M sodium cacodylate, pH 6.5, 200 mM NaCl, and equilibration with 0.5 ml reservoir solution, X-ray diffraction structure determination and analysis at 3.5 A resolution	Peptoniphilus asaccharolyticus

Crystallization (Comment)

Organism

purified recombinant wild-type and SeMet-labeled GDHs, hanging drop vapour diffusion method, at 20°C, protein in 10 mM Tris-HCl, pH 7.0, mixing of 0.002 ml of protein solution with 0.001 ml of reservoir solution containing 2.0 M ammonium sulfate, 0.1 M sodium cacodylate, pH 6.5, 200 mM NaCl, and equilibration with 0.5 ml reservoir solution, X-ray diffraction structure determination and analysis at 3.5 A resolution

Peptoniphilus asaccharolyticus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-glutamate + H2O + NAD+	Peptoniphilus asaccharolyticus	-	2-oxoglutarate + NH3 + NADH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Peptoniphilus asaccharolyticus	P28997	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamate + H2O + NAD+	-	Peptoniphilus asaccharolyticus	2-oxoglutarate + NH3 + NADH + H+	-	r

Synonyms

Synonyms	Comment	Organism
GDH	-	Peptoniphilus asaccharolyticus

Cofactor

Cofactor	Comment	Organism
additional information	NADP+ and NADPH are poor cofactors. The presence of an acidic residue at the P7 position, adjacent to the 2'-OH group, typically discriminates against NADP+. PaGDH contains a P7 glutamate and has high specificity for NAD(H), with a kcat/Km for NAD+ that is approximately 1000fold greater than that for NADP+	Peptoniphilus asaccharolyticus
NAD+	-	Peptoniphilus asaccharolyticus
NADH	-	Peptoniphilus asaccharolyticus