Application | Comment | Organism |
---|---|---|
molecular biology | GDH, in conjunction with NADH-glutamte synthase, contributes to the control of leaf glutamate homeostasis, an amino acid that plays a central signaling and metabolic role at the interface of the carbon and nitrogen assimilatory pathways | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
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Nicotiana tabacum | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
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leaf | - |
Nicotiana tabacum | - |
Synonyms | Comment | Organism |
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GDH | - |
Nicotiana tabacum |
glutamate dehydrogenase | - |
Nicotiana tabacum |
General Information | Comment | Organism |
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physiological function | transgenic tobacco plants overexpressing the two genes encoding the enzyme are generated. Using an in vivo real time 15 N-nuclear magnetic resonance (NMR) spectroscopy approach it is shown that, when the two GDH genes are overexpressed individually or simultaneously, the transgenic plant leaves do not synthesize glutamate in the presence of NH4+ when glutamine synthetase is inhibited. When the two GDH unlabeled substrates ammonium and glutamate are provided simultaneously with either (15N) glutamate or 15NH4+ respectively, it is found that the ammonium released from the deamination of glutamate is reassimilated by the enzyme glutamine synthase, suggesting the occurrence of a futile cycle recycling both ammonium and glutamate | Nicotiana tabacum |