Cloned (Comment) | Organism |
---|---|
ligated to NdeI/BamHI digested pET3a, expression in Escherichia coli BL21 (DE3) | Haloferax mediterranei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | activity with KCl slightly higher than with NaCl | Haloferax mediterranei | |
NaCl | - |
Haloferax mediterranei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47950 | - |
x * 54000, SDS-PAGE, x * 47950, sequence analysis | Haloferax mediterranei |
54000 | - |
x * 54000, SDS-PAGE, x * 47950, sequence analysis | Haloferax mediterranei |
290000 | - |
recombinant NAD-GDH | Haloferax mediterranei |
310000 | - |
wild-type NAD-GDH | Haloferax mediterranei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax mediterranei | Q977U6 | strain R4 (ATCC 33500) | - |
Haloferax mediterranei R-4 / ATCC 33500 | Q977U6 | strain R4 (ATCC 33500) | - |
Purification (Comment) | Organism |
---|---|
gel filtration, native enzyme 232fold purified, recombinant enzyme 5.4fold purified | Haloferax mediterranei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
89.3 | - |
purified recombinant enzyme | Haloferax mediterranei |
104 | - |
purified native enzyme | Haloferax mediterranei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH3 + NADH | - |
Haloferax mediterranei | L-glutamate + H2O + NAD+ | - |
r | |
2-oxoglutarate + NH3 + NADH | - |
Haloferax mediterranei R-4 / ATCC 33500 | L-glutamate + H2O + NAD+ | - |
r | |
L-glutamate + H2O + NAD+ | - |
Haloferax mediterranei | 2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
L-glutamate + H2O + NAD+ | - |
Haloferax mediterranei R-4 / ATCC 33500 | 2-oxoglutarate + NH3 + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, SDS-PAGE, x * 47950, sequence analysis | Haloferax mediterranei |
Synonyms | Comment | Organism |
---|---|---|
GDH, NAD-dependent | - |
Haloferax mediterranei |
NAD-GDH | - |
Haloferax mediterranei |
NAD-glutamate dehydrogenase | - |
Haloferax mediterranei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Haloferax mediterranei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Haloferax mediterranei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.3 | 9.5 | - |
Haloferax mediterranei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADPH | Haloferax mediterranei | |
NAD+ | - |
Haloferax mediterranei | |
NADH | - |
Haloferax mediterranei |