Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli TG1 cells | [Clostridium] symbiosum |
Protein Variants | Comment | Organism |
---|---|---|
D165N | residual 2% of wild-type activity when purified after expression in Escherichia coli at 37°C, cells induced at 8°C are 1000fold less active than that produced at 37°C, spontaneous deamidation, which depends on the residual catalytic machinery of the mutated GDH active site | [Clostridium] symbiosum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0056 | - |
NADH | mutant D165N | [Clostridium] symbiosum | |
0.0108 | - |
NADH | wild-type | [Clostridium] symbiosum | |
0.076 | - |
NAD+ | mutant D165N | [Clostridium] symbiosum | |
0.0862 | - |
2-oxoglutarate | mutant D165N | [Clostridium] symbiosum | |
0.125 | - |
2-oxoglutarate | wild-type | [Clostridium] symbiosum | |
0.14 | - |
NAD+ | wild-type | [Clostridium] symbiosum | |
2.66 | - |
L-glutamate | mutant D165N | [Clostridium] symbiosum | |
3.97 | - |
L-glutamate | wild-type | [Clostridium] symbiosum | |
49.4 | - |
NH4+ | mutant D165N | [Clostridium] symbiosum | |
62.8 | - |
NH4+ | wild-type | [Clostridium] symbiosum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Clostridium] symbiosum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity | [Clostridium] symbiosum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0003 | - |
mutant D165N overexpressed at 8°C | [Clostridium] symbiosum |
0.034 | - |
mutant D165N overexpressed at 37°C | [Clostridium] symbiosum |
0.047 | - |
mutant D165N overexpressed at 23°C | [Clostridium] symbiosum |
Storage Stability | Organism |
---|---|
-20°C | [Clostridium] symbiosum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH4+ + NADPH | - |
[Clostridium] symbiosum | L-glutamate + NADP+ | - |
? | |
L-glutamate + NAD+ + H2O | - |
[Clostridium] symbiosum | 2-oxoglutarate + NADH + NH3 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
[Clostridium] symbiosum |
glutamate dehydrogenase | - |
[Clostridium] symbiosum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
8 | 37 | decreasing temperature results in much lower enzyme activity | [Clostridium] symbiosum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
[Clostridium] symbiosum |