Literature summary for 1.4.1.2 extracted from

Goda, S.; Kojima, M.; Nishikawa, Y.; Kujo, C.; Kawakami, R.; Kuramitsu, S.; Sakuraba, H.; Hiragi, Y.; Ohshima, T.
Intersubunit interaction induced by subunit rearrangement is essential for the catalytic activity of the hyperthermophilic glutamate dehydrogenase from Pyrobaculum islandicum (2005), Biochemistry, 44, 15304-15313.

Search for more literature for 1.4.1.2

Activating Compound

Activating Compound	Comment	Organism	Structure
heating	at 90°C the activity of the recombinant enzyme increases to a level comparable to that of the native enzyme	Pyrobaculum islandicum
Urea	at 5 M the activity of the recombinant enzyme increases to a level comparable to that of the native enzyme, urea-induced activation of recombinant GDH is irreversible	Pyrobaculum islandicum

Application

Application	Comment	Organism
additional information	subunit rearrangement, i.e., a change in the quaternary structure of the hexameric recombinant GDH, is essential for activation of the enzyme	Pyrobaculum islandicum

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli (DE3)-codon plus-RIL cells	Pyrobaculum islandicum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.018	-	NAD+	recombinant, urea-activated enzyme	Pyrobaculum islandicum
0.019	-	NAD+	native enzyme	Pyrobaculum islandicum
0.021	-	NAD+	recombinant, heat-activated enzyme	Pyrobaculum islandicum
0.16	-	L-glutamate	native enzyme	Pyrobaculum islandicum
0.16	-	L-glutamate	recombinant, heat-activated enzyme	Pyrobaculum islandicum
0.18	-	L-glutamate	recombinant, urea-activated enzyme	Pyrobaculum islandicum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
47000	-	6 * 47000, SDS-PAGE	Pyrobaculum islandicum
280000	-	recombinant enzyme, gel filtration	Pyrobaculum islandicum
299000	-	recombinant, heat-activated enzyme, gel filtration	Pyrobaculum islandicum
337000	-	recombinant, urea-activated enzyme, gel filtration	Pyrobaculum islandicum

Organism

Organism	UniProt	Comment	Textmining
Pyrobaculum islandicum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pyrobaculum islandicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.51	-	native enzyme	Pyrobaculum islandicum
3.54	-	recombinant, urea-activated enzyme	Pyrobaculum islandicum
3.65	-	recombinant, heat-activated enzyme	Pyrobaculum islandicum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamate + H2O + NAD+	-	Pyrobaculum islandicum	2-oxoglutarate + NH3 + NADH + H+	-	r

Subunits

Subunits	Comment	Organism
hexamer	6 * 47000, SDS-PAGE	Pyrobaculum islandicum

Synonyms

Synonyms	Comment	Organism
GDH	-	Pyrobaculum islandicum
glutamate dehydrogenase	-	Pyrobaculum islandicum

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Release 2023.1 (January 2023)