Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetonitrile | activates | Pyrobaculum islandicum | |
guanidine hydrochloride | activates | Pyrobaculum islandicum | |
tetrahydrofuran | activates | Pyrobaculum islandicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
NADH | - |
Pyrobaculum islandicum | |
0.025 | - |
NAD+ | - |
Pyrobaculum islandicum | |
0.066 | - |
2-oxoglutarate | - |
Pyrobaculum islandicum | |
0.17 | - |
L-glutamate | - |
Pyrobaculum islandicum | |
9.7 | - |
NH4+ | - |
Pyrobaculum islandicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
6 * 36000, SDS-PAGE | Pyrobaculum islandicum |
220000 | - |
gel filtration | Pyrobaculum islandicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum islandicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrobaculum islandicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH4+ + NADPH | - |
Pyrobaculum islandicum | L-glutamate + NADP+ | - |
? | |
L-glutamate + H2O + NAD+ | - |
Pyrobaculum islandicum | 2-oxoglutarate + NH3 + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 36000, SDS-PAGE | Pyrobaculum islandicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Pyrobaculum islandicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
no loss of activity after 2 h | Pyrobaculum islandicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pyrobaculum islandicum |