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Literature summary for 1.4.1.2 extracted from
- A new class of glutamate dehydrogenases (GDH) (2000), J. Biol. Chem., 275, 39529-39542.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| AMP | stimulation | Streptomyces clavuligerus |  |
| asparagine | activates | Streptomyces clavuligerus | |
| aspartate | activates | Streptomyces clavuligerus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Streptomyces clavuligerus | |
| NADH | - |
Streptomyces clavuligerus | |
| NH4+ | - |
Streptomyces clavuligerus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 33 | - |
NH4+ | - |
Streptomyces clavuligerus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 183000 | - |
6 * 183000, SDS-PAGE | Streptomyces clavuligerus |
| 1100000 | - |
gel filtration | Streptomyces clavuligerus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces clavuligerus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces clavuligerus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21.2 | - |
- |
Streptomyces clavuligerus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + NH3 + NADH | - |
Streptomyces clavuligerus | L-glutamate + H2O + NAD+ | - |
r | |
| 2-oxoglutarate + NH4+ + NADPH | - |
Streptomyces clavuligerus | L-glutamate + NADP+ | - |
? | |
| L-glutamate + H2O + NAD+ | - |
Streptomyces clavuligerus | 2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 183000, SDS-PAGE | Streptomyces clavuligerus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Streptomyces clavuligerus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Streptomyces clavuligerus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Streptomyces clavuligerus | |
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Release 2023.1 (January 2023)
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