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Literature summary for 1.4.1.2 extracted from
- Purification, properties, and metabolic roles of NAD+-glutamate dehydrogenase in Clostridium botulinum 113B (1988), Arch. Microbiol., 150, 460-464.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoate) | - |
Clostridium botulinum |  |
| ADP | - |
Clostridium botulinum | |
| AMP | - |
Clostridium botulinum | |
| ATP | - |
Clostridium botulinum | |
| fumarate | amination | Clostridium botulinum | |
| glutamate | amination | Clostridium botulinum | |
| glutamine | amination | Clostridium botulinum | |
| H2O2 | - |
Clostridium botulinum | |
| Hg2+ | - |
Clostridium botulinum | |
| oxaloacetate | amination | Clostridium botulinum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42500 | - |
6 * 42500, SDS-PAGE | Clostridium botulinum |
| 250800 | - |
gel filtration | Clostridium botulinum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium botulinum | - |
113B | - |
| Clostridium botulinum 113B | - |
113B | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium botulinum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1092 | - |
above | Clostridium botulinum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, loss of activity overnight | Clostridium botulinum |
| 4°C, 3 days, stable | Clostridium botulinum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + H2O + NAD+ | - |
Clostridium botulinum | 2-oxoglutarate + NH3 + NADH + H+ | - |
? | |
| L-glutamate + H2O + NAD+ | - |
Clostridium botulinum 113B | 2-oxoglutarate + NH3 + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 42500, SDS-PAGE | Clostridium botulinum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
10 min, 40% loss of activity | Clostridium botulinum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Clostridium botulinum | |
| NADH | - |
Clostridium botulinum | |
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