Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | required for full activity | Pseudomonas sp. | |
dithiothreitol | can replace 2-mercaptoethanol | Pseudomonas sp. |
General Stability | Organism |
---|---|
unstable in the absence of 2-mercaptoethanol or dithiothreitol | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADPH | substrate inhibition above 1 mM | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
NADPH | - |
Pseudomonas sp. | |
15 | - |
pyruvate | - |
Pseudomonas sp. | |
75 | - |
methylamine | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36500 | - |
SDS-PAGE | Pseudomonas sp. |
77000 | - |
gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + methylamine + NADPH | Pseudomonas sp. | reverse reaction only to limited extend at very low rate | N-methyl-L-alanine + H2O + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
MS ATCC 25262 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.37 | - |
purified enzyme | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylamine + alpha-ketobutyrate + NADPH | 14% of activity with pyruvate | Pseudomonas sp. | glutamic acid + H2O + NADP+ | - |
? | |
methylamine + oxaloacetate + NADPH | 64% of activity with pyruvate | Pseudomonas sp. | aspartic acid + H2O + NADP+ | - |
? | |
pyruvate + methylamine + NADPH | reverse reaction only to limited extend at very low rate | Pseudomonas sp. | N-methyl-L-alanine + H2O + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36500, SDS-PAGE, gel filtration | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | 8.6 | - |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Pseudomonas sp. |